EGAGenomicsIllumina MiSeqResequencinghttps://ega-archive.org/studies/EGAS00001000430EGAEGA study EGAS00001000430Validation of AML Mutational ScreeningEGArestrictedRunx-1, aml, leukemia, Acute myelogenous leukemia, Leukemia, acute non lymphoblastic leukemia, Myeloid, Myeloblastic, Xaml1, Acute Myeloblastic Leukemia, susceptibility to, Acute Myeloid Leukemia, aml1., amlcr1, pebp2ab, AML, XAML, Acute, acute non lymphoblastic leukaemia, aml-1, ANLL, acute myeloid, aml1-evi-1, cbfa2, evi-1, acute myelogenousADD-87, Materials, AU023367, acute non lymphoblastic leukemia, Myeloid, Addresses, Ovhts, Gene, HtsRC, CG9325, Polymerase Chain Reactions, Inverse, Xt, 1B1, Mutations, anon-EST:Posey9, method, Inverse PCR, acute non lymphoblastic leukaemia, Add-hts, method used in an experiment, l(2)k14523, l(2)00634, acute myeloid, Ovhts-RC, Polymerase Chain, Runx-1, study, Leukemia, HTS-R1, Genetic, Acute Myeloblastic Leukemia, Inverse Polymerase Chain Reaction, HTS-RC, amlcr1, pebp2ab, aml1, XAML, Reaction, ANLL, Anchored PCR, l(2)01103, CG34197, HtsF, acute myelogenous, Add, leukemia, DmelCG43443, ADD, Hts-RC, Anchored Polymerase Chain Reaction, Cistrons, Dmel_CG9325, AML, add, htsRC, GLI3-190, CG43443, Genetic Materials, Dmel_CG34197, aml1-evi-1, cbfa2, evi-1, Bph, Genetic Material, PCR, aml, Acute myelogenous leukemia, Anchored, HTS, Hts, Reactions, Nested, l(2)k06121, Nested Polymerase Chain Reaction, Myeloblastic, Xaml1, adducin, GLI3FL, AI854843, susceptibility to, add-like, Acute Myeloid Leukemia, study protocol, Pdn, polymerase chain reaction, MiSeq., plan specification, Adducin, Acute, Comments, Material, aml-1, Manuscripts, Nested PCR, Cistron, Attention Deficit Hyperactivity Disorder, EST DfalseValidation of AML Mutational ScreeningWe recently worked-up a pulldown protocol for studying 21 genes recurrently mutated in AML (Study1770). Our manuscript is currently under revision and to address the reviewers' comments we need to validate some mutations by re-sequencing. In this add-on study we will be using PCR followed by MiSeq for this purpose.2021-04-23 20:10:07EGAS000010004309606EGAD00001000445EGAC00001000205