EGAGenomicsOtherhttps://ega-archive.org/studies/EGAS00001001568EGAEGA study EGAS00001001568Sequencing Data for Sample 51_Hf01_BlCM_CtEGArestrictedChIP-Chip, human being, DNS, T-cell surface antigen T4/Leu-3, Chromatin Immuno-precipitation, (Deoxyribonucleotide)n, Gene, CLIP-Seq, protein, broad, Assay for Transposase-Accessible Chromatin Using Sequencing, Deoxyribonucleic acids, Human, L3T4, Polynucleosome, Deoxyribonucleic Acid, Homo sapiens, Chromatin Immunoprecipitation Sequencing-Chip, establishment and maintenance of position, Gene Products, Ly-4, establishment and maintenance of substrate location, Low, Man, ChIA-PET, study, Chromatin Immunoprecipitation Sequencing Chip, thymus nucleic acid, me75, Chromatin Immuno precipitation Sequencing, Man (Taxonomy), Genomes, ChIP, Chromatin Immunoprecipitation Paired End Tag, Chromatin Immuno Precipitation Paired End Tag, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, Double Stranded, Deoxyribonucleic acid, proteins, DNA Methylomes, man, D17Mit170, T1, Sequencing, Chromatin Immunoprecipitation Paired-End Tag, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Double-Stranded DNA, (Deoxyribonucleotide)m, deoxyribonucleic acids, DNAn, associated, HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, establishment and maintenance of substance location, wide/broad, cou, T-cell surface antigen T4|Leu-3, Methylomes, DNAn+1, Modern, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, Proteins, ChIP Sequencing, Double-Stranded, Tl3, Tl2, ChIP-PET, Cell, ChIP-Exo, (Deoxyribonucleotide)n+m, single organism localization, Nucleosome, H3K4me3, Lr, native protein, T-cell differentiation antigen L3T4, Protein, establishment and maintenance of localization, Polynucleosomes, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, ds-DNA, desoxyribose nucleic acid, Methylome, Dinucleosome, distinct, Epigenomes, Protein., T-cell surface glycoprotein CD4, Chromatin Immunoprecipitation, CD4mut, Chromatin Immuno-precipitation Sequencing, single-organism localization, whole genome, ChIP Exonuclease, ChIP-Seq, Dinucleosomes, human, Assay for Transposase Accessible Chromatin Using Sequencing, Protein Gene Products, Gene Proteins, wide, localisation, Modern Man, Chromatin Immuno-Precipitation Paired-End Tag, ds DNA, Desoxyribonukleinsaeure, Bra, CD4, DNA, ChIP-Exonuclease, establishment and maintenance of cellular component location, DNA MethylomeLy-4, CD4, Cell., L3T4, CD4mut, H3K4me3, T-cell surface antigen T4/Leu-3, T-cell surface antigen T4|Leu-3, T-cell differentiation antigen L3T4, T-cell surface glycoprotein CD4falsereChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4+ memory T-CellsIdentification of genomic loci exhibiting the physical co-localization of two distinct DNA-associated proteins remains a technical challenge. To tackle this challenge we have developed a novel reChIP-seq approach that enriches for two distinct DNA-associated proteins in an unbiased and genome-wide manner. We illustrate the utility of our approach by identifying nucleosomes bivalently modified by H3K4me3 and H3K27me3 in primary human CD4+ central memory T-cells (TCMs). We unravel widespread bivalency of many unmethylated CpG-rich regions in TCMs overlapping with transcriptional start sites of developmental regulators. Furthermore, reChIP-seq uncovers two yet unobserved classes of bivalency, where either H3K4me3 or H3K27me3 is mixed with low but detectable bivalency. We provide evidence that bivalency is established by an interplay between genome and epigenome and thus attains stability. Our described reChIP-seq approach augments conventional ChIP-seq and is broadly applicable in the study of co-localization of DNA-associated proteins to unravel the intricate possibilities of combinatorial action.2017-07-26 15:39:27EGAS000010015689606EGAD00001002255EGAC00001000179