EGAGenomicsIllumina 450kOtherhttps://ega-archive.org/studies/EGAS00001001575EGAEGA study EGAS00001001575450kBangladeshEGArestrictedglicoproteinas, a glycoprotein, cord blood, glycated protein, O-Glycosylated, Thrombus, Materials, C-Glycosylated, multicellular organismal biosynthetic process., Blood, Proteins, Blood Clots, Gene, Fetal Bloods, Umbilical Cord Blood, DNA Methylations, Umbilical, fetal blood, Cistrons, Bloods, blood Clots, Cord, clot, Umbilical Cord Bloods, Thromboses, Glycoprotein, reduced, glycoproteins, subnumerary, Protein, Glycosylated Proteins, Genetic Materials, Platelet Activations, glycoproteine, present in fewer numbers in organism, Genetic Material, C-Glycosylated Proteins, glicoproteina, Glykoprotein, Umbilical Cord, single-organism biosynthetic process, N-Glycosylated, DNA methylation maintenance, Genetic, thrombotic disorder, Activation, N-Glycosylated Proteins, thrombus, Glycosylated, platelet activation, glycoproteines, thrombosis, Cord Bloods, INSDC_feature:gene, DNA methylation, decreased number, umbilical cord blood, O-Glycosylated Proteins, blood coagulation, decreased, Clots, Fetal, Prot/GlycoRes+, Material, Atherothrombosis, blood, Cord Blood, Platelet, Activations, Cistron, glycosylated protein, DNA, Glycosylated Protein, Methylations, Methylation, blood clot, Glykoproteine, Neoglycoproteins, Blood Clotglicoproteinas, dMelt, O-Glycosylated, Thrombus, Circulatory Systems, Materials, Other diseases of pericardium (disorder), Cardiovascular disease, determination, Other pericardial disease NOS (disorder), Blood, Other heart disease NOS (disorder), Cardiovascular disorder, RBC, PAPILLARY MUSCLE DIS NEC, DNA Methylations, sci, Cardiac Event, clefted, thrombocyte aggregation, [X]Other specified diseases of pericardium (disorder), clot, Thromboses, unspecified, 5 Methylcytosine, Glycoprotein, Disease of cardiovascular system (disorder), glycoproteins, Associations, Other heart disease NOS, AA959943, CVD, HOW, How, 5 Methylcytosine Monohydrochloride, 5-Methylcytosine Monohydrochloride, glycoproteine, Fs(3)Hor, l(3)j5D5, IKKg, C-Glycosylated Proteins, KEY, Key, multicellular organismal biosynthetic process, 24B, glicoproteina, Cardiovascular Diseases, Glykoprotein, single-organism biosynthetic process, DmelCG2684, N-Glycosylated, DNA methylation maintenance, red blood corpuscle, thrombus, entire life cycle, Moods, [X]Other forms of heart disease (disorder), hypoplasia, stru, Other ill-defined heart disease (disorder), Other diseases of endocardium, l(3)S053606, DNA methylation, NTef2, circulatory system disease, CG10293, Acts, Epigenomic, allergic reaction, l(3)j5B5, geographical area, Disorder of cardiovascular system, urinary aspects, Fetal, Prot/GlycoRes+, blood, Red, CG8624, ICP, Glycosylated Protein, Event, Cardiovascular Systems, blood clot, Neoglycoproteins, Blood Clot, Acta-2, a glycoprotein, close to, cord blood, 0904/17, Circulatory system disease NOS (disorder), Monohydrochloride, subdivided, life., Blood Clots, Actsk-1, Red Blood Cell, Disorder of circulatory system, Umbilical, fetal blood, disease of subdivision of hemolymphoid system, Fs(3)Sz11, Umbilical Cord Bloods, DmIKKgamma, SZ1, dIKK, Kenny, Adverse Cardiac Event, margin of safety, Genetic Materials, Red Blood Corpuscle, NOS, cholestasis, Other pericardial disease NOS, END, Genetic Material, region, CIRCULATORY DISEASE NOS, CARDIOVASC DIS, forked, Gravidic intrahepatic cholestasis, DmelCG8624, RICP, Blood Cell, divided, positional polypeptide feature, Epigenetic, life, Glycosylated, VEPH, red blood cell, IKK-gamma, Adverse Cardiac Events, Disorder of the circulatory system, thrombosis, [X]Other ill-defined heart diseases (disorder), umbilical cord blood, septate, early, Red Blood Corpuscles, DmelCG16910, Material, Red Blood Cells, Horka, Atherothrombosis, ILL-DEFINED HRT DIS NEC, intrahepatic cholestasis of pregnancy, CG2684, Erythrocyte, Fs(3)Horka, Platelet, anon-EST:Liang-2.39, Cistron, Blood Corpuscles, DNA, Epigenetics, Disease affecting entire cardiovascular system, Methylation, Cardiovascular Disorders, MELT, ASCVD, lifespan, 5-Methylcytosine, Cardiovascular system disease, C-Glycosylated, region or site annotation, CV system, P62, Other heart disease (disorder), Gene, Fetal Bloods, Other diseases of pericardium, BDPLT11, Other disorders of papillary muscle, Adverse, Bloods, blood Clots, dIKK-gamma, unspecified (disorder), anon-WO03014152.10, Disorder of cardiovascular system (disorder), [X]Cardiovascular disease, Cardiac, reduced, sensitive, DmIKK-gamma, Mood, DmF2, tiny, dmIKKgamma, IKK[[gamma]], Other ill-defined heart disease NOS (disorder), Blood Corpuscle, sensitivity, Unspecified circulatory system disorder, lod, study, Umbilical Cord, positional, urinary levels, Other diseases of endocardium (disorder), l(3)s2612, Genetic, [X]Other specified diseases of pericardium, entire lifespan, gravid, familial intrahepatic cholestasis of pregnancy, Aggregation, glycoproteines, Other heart disease, Mother, Other forms of heart disease, O-Glycosylated Proteins, Other specified pericardial disease NOS, cardiovascular disease, Melt, IKK, Herz und Gefaesssystem, DmelCG10293, Cord Blood, site, Disease of cardiovascular system, 1441/14, toxic potential, glycosylated protein, Methylations, Circulatory system disease NOS, Circulatory System, HHT1, GPVI, Glykoproteine, OTHER SEQUELAE OF MI NEC, small, Disease, glycated protein, Other ill-defined heart diseases, Edg, clone 2.39, Affects, not elsewhere classified, Proteins, familial recurrent intrahepatic cholestasis of pregnancy, Umbilical Cord Blood, Disease affecting entire cardiovascular system (disorder), qkr, pregnant adult, l(3)S090417, Cistrons, Other ill-defined heart disease, Cord, pregnant, near to, IKKgamma, pregnancy related cholestasis, Cardiac Events, KH93F, chemical analysis, Protein, Glycosylated Proteins, sequence, methylation, intrahepatic of pregnancy, glycoprotein 5, Other forms of heart disease (disorder), Other ill-defined heart disease NOS, Foods, PERICARDIAL DISEASE NEC, INSDC_feature:regulatory, who, Blood Cells, Other specified diseases of pericardium, Cardiovascular, thrombotic disorder, underdeveloped, N-Glycosylated Proteins, Dmikkgamma, [X]Other ill-defined heart diseases, Cord Bloods, [X]Other forms of heart disease, Who/How, Other sequelae of myocardial infarction, Lds, CG16910, primary structure of sequence macromolecule, pregnancy-related cholestasis, Cardiovascular Disease, CVS disease, Other specified pericardial disease NOS (disorder), circulatory system, Clots, gravidic intrahepatic cholestasis, approaches, vicinity of, qkr[93F], recurrent intrahepatic cholestasis of pregnancy, assay, Major Adverse Cardiac Events, GPIV, ORW1falsePrenatal lead exposure is associated with decreased cord blood DNA methylation of the glycoprotein VI gene involved in platelet activation and thrombus formationEarly-life lead exposure impairs neurodevelopment and later exposure affects the cardiovascular system. Lead has been associated with reduced global 5-methylcytosine DNA methylation, suggesting that lead toxicity acts through epigenetic mechanisms. The objective of this study is to clarify how early life lead exposure alters DNA methylation of specific genes, using an epigenomic approach. We measured lead concentrations in urine (gestational week, GW, 8) and erythrocytes (GW 14), using ICP-MS, for 127 pregnant mothers recruited in the MINIMat food and supplementation cohort in rural Bangladesh. Cord blood DNA methylation was analyzed with the Infinium HumanMethylation450K BeadChip and top sites were validated by methylation-sensitive high-resolution melt curve analysis. Maternal urinary lead concentrations (divided into quartiles) showed significant (after adjustment for FDR) inverse associations with methylation at nine CpGs. Three of these sites were in the 5’ end, including the promoter, of glycoprotein IV (GP6); cg18355337 (q=0.029, β=-0.30), cg25818583 (q=0.041, β=-0.18) and cg23796967 (q=0.047, β=-0.17). The methylation in another CpG site in GP6 was close to significant (cg05374025, q=0.057, β=-0.23). The erythrocyte lead concentrations (divided into quartiles) were also inversely associated with CpG methylation in GP6, although this was not statistically significant after FDR-adjustments. Eight CpG sites in GP6 constituted a differentially methylated region in relation to urinary lead (p=0.005, q=0.48) and erythrocyte lead (p=0.007, q=0.46). In conclusion, we found that moderate prenatal lead exposure appear to epigenetically affect GP6, a key component of platelet aggregation and thrombus formation, suggesting a novel link between early lead exposure and cardiovascular disease later in life.2017-07-26 15:39:27EGAS000010015759606EGAD00010000946EGAC00001000313