EGAGenomicsIllumina MiSeqCancer Genomicshttps://ega-archive.org/studies/EGAS00001001591EGAEGA study EGAS00001001591RNAseq of ribosomal footprintsEGArestrictedmalignant Growth, d230, Materials, cell type cancer, SCF receptor activity, rectal adenocarcinoma, Gene, dTAFII250, Xkl-1, krk1., EfW1, dmTAF[[II]]230, KL receptor activity, Gsfsco1, scfr, dmTAF1, Taf230, SCO5, Mood, SCO1, rectum adenocarcinoma, Gsfsow3, neoplasm, SCFR, Gsfsco5, malignant tumour, SOW3, TAF250, Normalities, average, Taf200, Fdc, dTAF[[II]]250, Genetic, TFIID TAF250, cel, cell, adenocarcinoma of rectum, Moods, adenocarcinoma of the rectum, neoplasm (disease), Taf1p, W, CA, dTAF250, malignant neoplasm, Bs, TAF, Sl, proto-oncogene c-Kit, malignancy, dTAF[[II]]230, TAF[[II]]250, malignant, Affects, TAF200, l(3)84Ab, Tr-kit, BG:DS00004.13, Normalcy, TAFII-250, TAF250/230, Cistrons, Cell, results, dTAF230, read, PBT, TAFII250, MT, KIT ligand receptor activity, p230, malignant neoplasm (disease), organ system cancer, TAF[[II]]250/230, kl1-A, Genetic Materials, TFIID, adenocarcinoma - rectum, KIT, Genetic Material, XKrk1, Taf[[II]]250, tyrosine-protein kinase Kit, pbt, c-KIT, primary cancer, TAF[[II]]230, Normality, TAF[II]250, kit, CD117, CG17603, TAF[[II]], malignant tumor, Health, c-kit, DmelCG17603, Taf250, C-Kit, malignant neoplastic disease, Material, SR3-5, Indexes, Ssm, xkl-1, Cistron, cancer, TAF230, TAF1Total RNA Sequencing, Ribosome, Ribosomal, RNA-seq (transcriptome sequencing), RNAseq, Whole-Transcriptome Sequencing, Whole Transcriptome Sequencing, WHOLE TRANSCRIPTOME SEQUENCING, Whole Transcriptome Shotgun Sequencing, RNA Sequencing, Transcriptome Sequencing, WTSS, RNA-Seq, Total RNA-Seq, ribosome.0.00.00.00.00.0falseRNAseq of ribosomal footprintsCancer genes can affect ribosomal RNA processing and this can underlie their essentiality to cells, making them cell-essential in the same way as ribosomal genes themselves. We want to confirm this, in order to understand the results of our CRISPR drop-out screens.
NOTE FROM BESPOKE TEAM: Run a single read 1 (forward read) of 30 bases, then an index 1 read as normal. This would fit a 50cycle kit2017-07-26 15:39:25EGAS000010015919606EGAD00001001930EGAC00001000205