{"database":"EGA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"study_type":["Cancer Genomics"],"full_dataset_link":["https://ega-archive.org/studies/EGAS00001002132"],"host":["EGA"],"description":["EGA study EGAS00001002132"],"dataset_title":["The Causes of Clonal Blood Cell Disorders Study - SCOR (2018-04-19)","Reliable detection of somatic mutations in single DNA molecules"],"category":["restricted"],"repository":["EGA"],"additional_accession":[]},"is_claimable":false,"name":"The Causes of Clonal Blood Cell Disorders Study   SCOR","description":"We will take a bone marrow aspirate and peripheral blood samples from a healthy patient aged around 60, and use flow cytometry to isolate 100 HSCs, 50 MEPs, and 50 GMPs. We will grow these up into colonies, then whole genome sequence each colony. Somatic mutations will act as a unique barcode for each clone. We will then design a panel for targeted resequencing of the mutations that we find. It will then be possible to look for these mutations in the peripheral blood over several years, to see the dynamics of how HSCs contribute to the peripheral blood in health.","dates":{"updated":"2022-11-30 11:22:01"},"accession":"EGAS00001002132","cross_references":{"TAXONOMY":["9606"],"EGA":["EGAD00001004086","EGAD00001006595","EGAC00001000000"]}}