EGAGenomicsIllumina HiSeq 2500, ILLUMINACancer Genomicshttps://ega-archive.org/studies/EGAS00001002257EGAEGA study EGAS00001002257The Causes of Clonal Blood Cell Disorders Study - SCOR_Custom (2018-04-19)EGArestrictedblood cell, haematopoietic cell, Blood Cell, other disease, hemopoietic cell, human disease, Blood, disorders, haemopoietic cell, study., medical condition, Cell, non-neoplastic, Corpuscle, disease, diseases, Cells, Corpuscles, disease or disorder, condition, disorder, diseases and disorders, Homo sapiens disease, Blood Corpuscles, Blood Corpusclel(1)Ab, Act5c, l(1)G0010, 2410041A17Rik, ACTG, ACTE, Blood, ACT5C, CG12051, fs(1)M34, Serpin A3, ES2-2, Bach, csp2, Act5, l(1)G0420, Mutations, DmelCG12051, Fluorescence-Activated, l(1)G0025, Fluorescence-Activated Cell Sortings, Microfluorometry, Marrow, medulla ossea, PBMC, HEL-176, CG4601, Cell growth-inhibiting gene 24|25 protein, Yellow, Flow Microfluorimetry, Cell Sortings, Cytofluorometry, DmelCG9242, Normalities, A, average, C, act5C, cyt5C, GMPS, Fluorescence Activated Cell Sorting, DHO, Flow Cytofluorometries, Microfluorometries, BRWS2, CG18572, P174, Red Marrow, fs(1)829, Act42a, anon-EST:fe2D2, 1700027G07Rik, act 42A, Clients, DmelCG18572, Flow, gua2, Ac5C, Red, Flow Microfluorometries, CG4027, Cytometry, ACT, Act, T11, anon-WO0118547.193, Act-5C, Flow Cytofluorometry, Cte-II, Cytometries, Sortings, Normalcies., l(1)G0177, Cell Sorting, Individual Health, 42A, Fluorescence-Activated Cell Sorting, CPS, l(1)G0330, whole blood, Actin/BAP47, CTE-II, AACT, dJ393D12.2, CTE-IIa, act, Ach1, hBACH, DNA barcode, ACH1, Flow Cytometries, Normalcy, LACH1, Client, act42A, Microfluorimetry, GIG25, CAD, Gmps, medulla ossium, Su(b), Alpha-1-antichymotrypsin His-Pro-less, beta-actin, AFFX-Dros-ACTIN_M_r_at, GIG24, M32055, DFNA26, Act42, actin, Individual, ACTA3, Flow Microfluorometry, LACH, GAT, DFNA20, l(1)G0079, Yellow Marrow, Cytofluorometries, DmelCG4027, Peripheral Blood, Normality, medullary bone, medulla of bone, PYR1, l(1)G0117, VSCM, anon-WO0118547.204, act 5C, Actin, patient, whole genome, Fluorescence-Activated Cell, beta-actin/Bap47, Reticuloendothelial System, l(1)G0486, l(2)10523, l(1)G0245, actin5C, Health, l(1)G0009, Patient, ACTL3, Lach1, CG9242, PBMCs, Sorting, DRORUD, BAP47, ACTSG, BACH, Bone, Bap47, Elderly, Actin5CfalseThe Causes of Clonal Blood Cell Disorders Study SCOR CustomWe took a bone marrow aspirate and peripheral blood samples from a healthy patient aged around 60, and use flow cytometry to isolate 100 HSCs, 50 MEPs, and 50 GMPs. We grew these up into colonies, then whole genome sequenced each colony. Somatic mutations act as a unique barcode for each clone. We have designed a panel for targeted resequencing of the mutations that we find. We are now looking for these mutations in the peripheral blood, to see the dynamics of how HSCs contribute to the peripheral blood in health.2018-04-24 12:51:15EGAS000010022579606EGAD00001004087EGAC00001000000