EGAGenomicsOtherhttps://ega-archive.org/studies/EGAS00001003064EGAEGA study EGAS00001003064Whole exome and transcriptome sequencing of gastric cancer patientsEGArestrictedProliferating, POP2, Pop2, Neoplasms, Nurf-55, p55, RbAp48, Gene, CAF1A, Prognostic Factors, CAF1B, p55 CAF1, T2G17_18, Cac1p, P150, dCAF-1, Stomach Neoplasms, dCAF-1 p55, malignant fundus of stomach neoplasm, NURF, NEOPL STOMACH, DmelCG5684, Caf1b, Caf1a, Chaperone, DmelCG4236, T2G17.18, Ca fundus - stomach, hCAF1, stomach neoplasm, malignant neoplasm of fundus of stomach, Familial Diffuse, histone chaperone, CAF-Ip60, malignant tumor of fundus of stomach, pop2, tumor of the stomach, gastric fundus cancer, gastric neoplasm, dNURF, stomach tumor, caf1, Prognoses, AL024058, proliferating, stomach tumour, CAF-IP60, Gastric Cancer, xp150, malignant neoplasm of body of stomach, gastric cancer, AL023013, malignant tumour of fundus of stomach, d-CAF1, Cancer of Stomach, 154659_at, Cancer of the Stomach, CAF1-55, Ca body - stomach, Nurf, Cancer, regulation of gene product expression., CAF-1, tumour of stomach, CALIF, CAF-I p60, Stomach Neoplasm, Caf1/p55, Chaperones, GASC, CG4236, Regulation of Gene Expression, Caf-1, CAF1, Caf1, ATCAF1, CG5684, Factor, neoplasm of the stomach, Caf1p55, fundus of stomach cancer, Histone, cancer of fundus of stomach, Gastric cancer, gastric tumor, STOMACH NEOPL, 55, Gene Action, Stomach Cancers, hCAF-1, Gene Expression, NURF-55, C76145, Expression Regulation, p55CAF1, S(ls)3, Neoplasm, Gastric Neoplasms, gene regulation, chaf1a-b, Gastric, Stomach Cancer, AU022737, Nurf 55, Factors, gastric tumour, stomach neoplasm (disease), caf-1, Prognostic, Histone Chaperone, Gastric Cancers, ATP-dependent H3-H4 histone complex loader activity, Cancers, Gastric Neoplasm, regulation of protein expression, Stomach, Gene Action Regulation, p55/NURF-55, CAF1P150, Proliferation, neoplasm of stomach, NEOPL GASTRIC, caip150, MPP7, 2600017H24Rik, tumor of stomach, P55, NURF55, MSI1/RbAp48/CAC3/LIN-53, caf1 p55, CAF1p55, tumour of the stomach, Regulation, Nurf55, MPHOSPH7, ca greater curvature of stomach, CAF1P60, GASTRIC NEOPL, Prognostic FactorPOP2, dp53, Pop2, tumor suppressor, Metabolic Concepts, RbAp48, A4, Embden-Meyerhof, CAF1A, CAF1B, T2G17_18, P150, Tumor, Stomach Neoplasms, malignant fundus of stomach neoplasm, Caf1b, Cell Cycle-Transition Points, Caf1a, cell cycle regulator, Disease Free, T2G17.18, hCAF1, Oxidative, Associations, histone chaperone, bbl, responsivity, Cell Cycle Transition Points, pop2, Metabolism Concept, gastric neoplasm, Non Polyadenylated, gamma sarcoglycan, BCC7, catabolism, beta-Tub6D, Tissue, dmp53, T, Pathways, 1422/04, metabolic process resulting in cell growth, D17Mit170, malignant neoplasm of body of stomach, Intrinsic Pathway Apoptosis, Dp53, d-CAF1, Role Concepts, Cell Cycle, CG17117, p50/tubulin, single organism signaling, Tumors, CAF-1, CG10873, Pathway, GASC, Polyadenylated, metabolism resulting in cell growth, Regulation of Gene Expression, Caf-1, anaerobic glycolysis, Tl3, antibodies, Embden-Meyerhof-Parnas, Tl2, Ki67, STOMACH NEOPL, Embden-Meyerhof-Parnas Pathway, NURF-55, Benign, Role Concept, signaling (initiator) caspase activity, induction of apoptosis, arrest of mitotic cell cycle progression, S(ls)3, Role, Cellular, sarcoglycan, secretion, bfy, Caspase-Dependent, HTH, Hth, Stomach Cancer, Polyadenylated Messenger RNA, AU022737, Phosphorylation, Non Polyadenylated mRNA, gastric tumour, protein_coding_transcript, death rate, CG3401, Benign Neoplasms, beta3Tub, beta3TUB, Controls, Non-Polyadenylated mRNA, regulation of protein expression, Malignant Neoplasms, Intrinsic Pathway Apoptoses, DTB3, Cycle-Transition Point, B cell receptor accessory molecule complex, glycolysis, p55/NURF-55, 35kD dystrophin-associated glycoprotein, Proliferation, 2600017H24Rik, B lymphocyte receptor complex, tumor of stomach, Extrinsic Pathway, bhy, other neoplasm, CG31325, Poly(A) RNA, Embden-Meyerhof Pathways, beta[[3]]-Tub, DmelCG17117, heterogeneity., Processes, Neoplasms, Cell Cycle Control, 143391_i_at, Cardias, beta3 TU, Metabolic Processes, protein-containing complex, LFS1, dCAF-1 p55, l(3)05745, regulation of gene product expression, Ki-67, DmelCG5684, DmelCG4236, Gene Products, CAF-Ip60, malignant tumor of fundus of stomach, anon-EST:Liang-2.13, Number Growth, MAM, gamma-SG, dNURF, caf1, proliferating, Metabolic Concept, messenger RNA, CAF-IP60, Gastric Cancer, Neoplasias, template RNA, Oxidative Phosphorylations, Classic Apoptosis, Checkpoint, beta[[3]]-tubulin, negative regulation of cell cycle arrest, Cancer, Apoptosis, tumour of stomach, RNA, CAF-I p60, Dmbeta3, Stomach Neoplasm, Malignant Neoplasm, cou, Caf1/p55, 35 kDa dystrophin-associated glycoprotein, Cell Cycle-Transition Point, degradation, CG4236, prac, Proteins, beta3t, Phosphorylations, Messenger RNA, CAF1, Caf1, CG5684, Histone, cancer of fundus of stomach, Cell, SGCG, Concept, Stomach Cancers, positive regulation of cell cycle arrest, C76145, Lr, Checkpoints, native protein, Poly(A)+ mRNA, p53/tubulin, Neoplasm, INSDC_feature:mRNA, gene regulation, Cell Cycle Arrests, Gastric, metabolism, Metabolic Phenomenon, CG33336, multicellular organism metabolic process, DMDA1, apoptosis, underdeveloped, mRNA, D-p53, Dm-P53, Cancers, beta3, dtl, Cell Cycle Checkpoint, mitotic cell cycle arrest, Tub, Classic Apoptoses, Gene Action Regulation, Gene Proteins, signalling process, CAF1P150, betatub60D, Point, Bra, commitment to apoptosis, Polyadenylated mRNA, tumour of the stomach, Nurf55, Neoplasia, Anabolism, biochemical pathways, MGC130048, Metabolic Process, MIB-1, B-cell receptor complex, protein, p55 CAF1, betaTub3, Embden Meyerhof Parnas Pathway, Tp53, Cell Growth in Number, Arrests, NURF, NEOPL STOMACH, Chaperone, DMP53, Extrinsic Pathway Apoptoses, malignant neoplasm of fundus of stomach, Familial Diffuse, Roles, Cell Number Growth, Concepts, Dmp53, Embden-Meyerhof Pathway, tumor of the stomach, protein aggregate, gastric fundus cancer, Phenomenon, 1323/07, me75, Growth, AL024058, membrane bound, Polyadenylated Messenger, hypoplasia, DmP53, xp150, T1, B3t, DmelCG3401, PPP1R105, malignant tumour of fundus of stomach, Classic, morbidity-free survival, Classical Apoptosis, gamma-sarcoglycan, biotransformation, Malignancies, Cancer of the Stomach, Cell Cycle Arrest, B-lymphocyte receptor complex, CAF1-55, Catabolism, Ca body - stomach, Nurf, CALIF, Caspase-Dependent Apoptosis, Process, SG-gamma, neoplasm of the stomach, results, 55, Gene Action, 3t, hCAF-1, KIA, Gene Expression, Expression Regulation, Intrinsic Pathway, p55CAF1, Extrinsic Pathway Apoptosis, Gastric Neoplasms, chaf1a-b, Tub60D, beta-tub, Arrest, Disease Free Survival, Nurf 55, stomach neoplasm (disease), caf-1, cessation of cell cycle, DmelCG33336, Heterogeneity, Histone Chaperone, Control, D630048A14Rik, gamma (35kDa dystrophin-associated glycoprotein), Embden-Meyerhof pathway, Stomach, MIB-, hth1, immunoglobulin, DMDA, hth2, induction of apoptosis by p53, Patient, neoplasm of stomach, caip150, MPP7, P53, Cell Number, regulation of cell cycle arrest, P55, p44, microarray, MSI1/RbAp48/CAC3/LIN-53, Classical, Regulation, Apoptoses, MPHOSPH7, ca greater curvature of stomach, betaTub, Proliferating, SGCG_HUMAN, p50, p53, Nurf-55, caspase-dependent programmed cell death, p55, Benign Neoplasm, Caspase Dependent Apoptosis, Gene, arrest of cell cycle progression, Cac1p, dCAF-1, Malignant, TYPE, DAGA4, antibody, BCR complex, Ca fundus - stomach, stomach neoplasm, reduced, termination of cell cycle, Messenger, Metabolism, Survival, Genetic heterogeneity, modified Embden-Meyerhof pathway, 35DAG, Low, tiny, Type I, SCG3, immunoglobulin complex, Metabolism Phenomena, stomach tumor, study, reactivity, clone 2.13, Malignancy, D.m.BETA-60D, stomach tumour, apoptosis activator activity, respiratory-chain phosphorylation, gastric cancer, cell proliferation, Trp53, AL023013, time of survival, Cancer of Stomach, Clients, heterogeneity, 154659_at, betaTub60C, Disease-Free Survival, beta3-tubulin, Cell Cycle-Transition, TRP53, small, beta-Tub60D, Multiplication, Dm-HTH, Cellular Proliferation, Embden Meyerhof Pathway, Chaperones, cell cycle arrest, protein complex, BETA 60D, beta3-Tub, ATCAF1, Caf1p55, fundus of stomach cancer, Client, Gastric cancer, gastric tumor, LGMD2C, Xp53, Metabolic Phenomena, Metabolism Concepts, survival, Programmed Cell Death, Protein, Phenomena, Polyadenylated RNA, beta[[3]] tubulin, opsonin activity, Poly(A)+ RNA, biodegradation, Metabolic, Poly(A) Tail, Cell Cycle Controls, Gastric Cancers, beta60C, Non-Polyadenylated, ATP-dependent H3-H4 histone complex loader activity, l(3)86Ca, patient, Gastric Neoplasm, Embden-Meyerhof-Parnas pathway, Protein Gene Products, Meis1, NEOPL GASTRIC, SCARMD2, Cell Multiplication, B cell receptor activity, Disease-Free, NURF55, response, caf1 p55, CAF1p55, CAF1P60, GASTRIC NEOPLfalseHistone chaperone CHAF1A promotes proliferation and tumorigenicity in gastric cancer and impacts prognosis via context-depedent regulation of gene expressionThe histone chaperone CHAF1A is associated with some tumors, but its mechanisms in tumor biology remain elusive. Gastric cancer (GC) has significant heterogeneity among patients, and novel prognostic markers and therapeutic targets may improve its outcomes. The role of CHAF1A in GC is unknown. In this study, we demonstrated that CHAF1A was overexpressed in GC tissues accompanied by low expression of wild-type P53 and high expression of the proliferation marker MKI67. In vitro, CHAF1A knockdown inhibited cell proliferation, induced cell cycle arrest and promoted apoptosis, while CHAF1A overexpression had contrary effects. In vivo, CHAF1A knockdown inhibited tumorigenicity of GC cells. A microarray assay with CHAF1A inhibition and a PathScan signaling antibody array with CHAF1A overexpression showed that CHAF1A inhibited the P53 pathway, activated stress response and induced glycolytic metabolism. In 665 patients, the expression level of CHAF1A protein was an independent predictor for overall survival and disease-free survival in non-cardia GC and also had survival associations in patients with small or perineural invasion-negative tumors. Tumor mRNA analyses based on next-generation sequencing in patients indicated that CHAF1A promoted the Warburg effect by depressing oxidative phosphorylation and increasing glycolysis. Furthermore, the heterogeneous clinical significance of CHAF1A among patient subgroups was associated with its differential regulation of gene expression involved with glycolysis and cell proliferation and survival, indicating that the role of CHAF1A is depended on patient characteristics. These results reveal critical roles and clinical values for CHAF1A in GC. CHAF1A may have context-dependent effects in GC, which increases understandings for GC heterogeneity.2020-07-16 15:39:02EGAS000010030649606EGAD00001004164EGAC00001000598