EGAGenomicsOtherhttps://ega-archive.org/studies/EGAS00001003513EGAEGA study EGAS00001003513Comparison of 3 protocols for deriving pancreatic progenitors from hPSCs (RNA-seq)Comparison of protocols for deriving pancreatic progenitors from hPSCs (ATAC-seq)restrictedEGASoluble, Ribonucleic, Insulin B Chain, Materials, postnatal development., determination, Stem Cells, DELTA AND GAMMA, insuline humaine, growth and development, betaTub1, dmTAF[[II]]230, B1t, human insulin, Non Polyadenylated, RNA Gene Products, ChIA-PET, Chromatin Immunoprecipitation Sequencing Chip, insulin, Chromatin Immuno precipitation Sequencing, Man (Taxonomy), kus, beta1tub, TFIID TAF250, CG9277, cel, ChIP, ATAC-seq, Chromatin Immuno Precipitation Paired End Tag, T, beta-particle, Chromatin Immunoprecipitation Paired-End Tag, 1t, insulin human, e, e-, beta1Tub, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, insulinum humanum, dTAF[[II]]230, Regular, BETA 56D, ribose nucleic acid, Modern, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, ribonucleic acids, TAF200, TAFII-250, TAF250/230, ChIP-PET, Regular Insulin, insulin (recombinant), TAFII250, l(1)G0108, Stem Cell, Ribonukleinsaeure, pentosenucleic acids, Genetic Materials, FLOWERING TIME CONTROL PROTEIN FCA ALPHA, Ribonucleic acids, Soluble Insulin, Genetic Material, Acid, beta-tub, Beta <eudicots>, ATAC-seq assay, insulina humana, FCAALL.331, ChIP Exonuclease, beta56D, DmelCG9277, study protocol, CG17603, TAF[[II]], human, beta1-tub, beta(-), DTB2, beta-Tub, Taf250, Material, SR3-5, i6, DL4180C, Cistron, beta-Tub56D, ChIP-Exonuclease, humans, betaTub, Specbeta, TAF230, beta1-Tubulin, ChIP-Chip, d230, human being, Chromatin Immuno-precipitation, e(-), Gene, CLIP-Seq, dTAFII250, pancreas, Insulin B, Assay for Transposase-Accessible Chromatin Using Sequencing, electron, EfW1, Human, method, Homo sapiens, B-spec, dmTAF1, Taf230, betaspec, method used in an experiment, Insulin, Chromatin Immunoprecipitation Sequencing-Chip, Gene Products, Pluripotent Stem Cell, Man, TAF250, Chain, study, Taf200, betatub(56D), dTAF[[II]]250, Genetic, cell, Chromatin Immunoprecipitation Paired End Tag, Elektron, beta[[1]] tubulin, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, beta1t, Taf1p, Sequencing, Non-Polyadenylated RNA, anon-EST:fe1B3, dTAF250, Iletin, Insulin recombinant, SPEC8, CG5870, TAF, HITS-CLIP, beta-tubulin56D, Spec-beta, RNA, Dmbeta1, TAF[[II]]250, ChIP Sequencing, Tubulin, l(3)84Ab, RNS, BG:DS00004.13, Pluripotent, i168, Cistrons, Cell, ChIP-Exo, dTAF230, development, Sodium Insulin, yeast nucleic acid, p230, chemical analysis, Insulin A Chain, TAF[[II]]250/230, betaSpec, TFIID, Sodium, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Novolin, b-Spec, Taf[[II]]250, ribonucleic acid, DmelCG5870, l(1)G0074, Exubera, l(1)G0198, TAF[[II]]230, distinct, negatron, beta[[1]]-tubulin, Non Polyadenylated RNA, postnatal growth, Non-Polyadenylated, b spectrin, Chromatin Immunoprecipitation, beta1, Chromatin Immuno-precipitation Sequencing, TAF[II]250, Ribonucleic Acid, ChIP-Seq, Assay for Transposase Accessible Chromatin Using Sequencing, beta, plan specification, Tub, DmelCG17603, beta-spec, Modern Man, Chromatin Immuno-Precipitation Paired-End Tag, BETA, assay, growth, Spec, TAF1HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Chromatin Immunoprecipitation Sequencing Chip, ChIP-Chip, Chromatin Immuno precipitation Sequencing, Chromatin Immuno-precipitation, ATAC-seq assay, ChIP, Chromatin Immunoprecipitation Paired End Tag, ATAC-seq, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, Chromatin Immuno Precipitation Paired End Tag, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, ChIP Sequencing, RNA-seq, Chromatin Immunoprecipitation, CLIP-Seq, Chromatin Immuno-precipitation Sequencing, Assay for Transposase-Accessible Chromatin Using Sequencing, ChIP Exonuclease, ChIP-PET, ChIP-Seq, Sequencing, ChIP-Exo, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, ChIA-PET., Chromatin Immuno-Precipitation Paired-End Tag, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Chromatin Immunoprecipitation Sequencing-Chip, Whole Transcriptome Shotgun Sequencing, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, ChIP-ExonucleasefalseComparison of 3 protocols for deriving pancreatic progenitors from hPSC with RNA-seq and ATAC-seqSeveral distinct differentiation protocols for deriving pancreatic progenitors (PP) from human pluripotent stem cells have been described but it remains to be shown how similar the PP are across protocols, and how well they resemble their in vivo counterparts. Here we evaluated three differentiation protocols; performed RNA and ATAC-seq on isolated PP derived with these, and compared them to fetal human pancreas populations. This enabled us to define a shared transcriptional and epigenomic signature of the PP, including several genes not previously implicated in pancreas development. Furthermore, we identified a significant and previously unappreciated cross-protocol variation of the PP through multi-omics analysis and demonstrate how such information can be applied to refine differentiation protocols for derivation of insulin-producing beta-like cells. Together, our study highlights the importance of a detailed characterization of defined cell populations derived from distinct differentiation protocols, and provides a valuable resource for exploring human pancreatic development.2019-11-28 18:10:03EGAS000010035139606EGAD00001004823EGAD00001004824EGAC00001000818EGAC00001000335