{"database":"EGA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"study_type":["Other"],"full_dataset_link":["https://ega-archive.org/studies/EGAS00001003848"],"host":["EGA"],"description":["EGA study EGAS00001003848"],"dataset_title":["Long-read sequencing of prostate adenocarcinoma metastatic to left axillary lymph node. Data used to support Figure 6 in Pubmed ID 32025007 \"Pan-Cancer Analysis of Whole Genomes Consortium.\" Nature 2020 578:8293.","Woodcock et al TenMenDeep Study EGA Dataset A","Woodcock et al TenMenDeep Study EGA Dataset B"],"category":["restricted"],"repository":["EGA"],"name_synonyms":["Fresh, other disease, DNS, Fresh Frozen Plasma, (Deoxyribonucleotide)n, Prostate Neoplasms, Effects, Neoplasms, Blood, Serum, Deoxyribonucleic acids, Long Term, metastatic prostate cancer, Cerebrospinal, cerebral spinal fluid, Deoxyribonucleic Acid, diseases, Prostatic Cancers, disease or disorder, Cerebro Spinal, liquor cerebrospinalis, diseases and disorders, Cancer of Prostate, Effect, Prostate Cancers, Fresh Frozen Plasmas, Fresh Frozen, Blood Plasma, thymus nucleic acid, human disease, metastatic prostate carcinoma, Longterm, Double Stranded, Deoxyribonucleic acid, 163., Long-Term, non-neoplastic, Prostatic Neoplasm, Cancer of the Prostate, Clients, Blood Plasmas, Prostatic, disorder, Homo sapiens disease, Double-Stranded DNA, Long-Term Effect, (Deoxyribonucleotide)m, deoxyribonucleic acids, DNAn, Long-Term Effects, plasma, prostate carcinoma metastatic, Cancer, hereditary prostate cancer, Cerebro Spinal Fluid, cancer of the prostate, Prostates, Frozen Plasma, DNAn+1, familial, disorders, Fluids, Longterm Effect, Cerebrospinal Fluids, prostate cancer metastatic, medical condition, spinal fluid, Double-Stranded, Blood Serum, Plasmas, Client, Spinal Fluid, (Deoxyribonucleotide)n+m, Cerebro Spinal Fluids, Frozen Plasmas, Spinal Fluids, Fluid, male prostate, Prostatic Cancer, Long Term Effects, Diseases, Neoplasm, condition, NOS, ds-DNA, desoxyribose nucleic acid, Plasma, Body Fluid, portion of plasma, Cancers, CSF, Cerebro, cancer of prostate, Body, Prostate Neoplasm, Longterm Effects, disease, metastatic, Patient, ds DNA, Desoxyribonukleinsaeure, prostate cancer, PC, DNA, Prostate Cancer, prostate, Serums, Prostate, prostata"],"description_synonyms":["Fresh, Forms, other disease, Military Uniform, DNS, Fresh Frozen Plasma, Prostate Neoplasms, determination, (Deoxyribonucleotide)n, Metastasis, Effects, Neoplasms, Blood, Benign Neoplasm, Neoplasm Metastases, neoplasm metastasis, Tumor, Serum, Malignant, Military Uniforms, Long Term, cancer metastasis, Cerebrospinal, cerebral spinal fluid, diseases, Deoxyribonucleic Acid, susceptible, Prostatic Cancers, Cerebro Spinal, disease or disorder, liquor cerebrospinalis, diseases and disorders, Cancer of Prostate, Effect, Prostate Cancers, tumor cell migration, Fresh Frozen Plasmas, Fresh Frozen, Blood Plasma, Nurse Uniform, human disease, thymus nucleic acid, Malignancy, Longterm, occurrence, prevalence, Double Stranded, metastasis, Deoxyribonucleic acid, Long-Term, Military, non-neoplastic, Neoplasias, Prostatic Neoplasm, Cancer of the Prostate, Clients, Blood Plasmas, Prostatic, disorder, Homo sapiens disease, Malignancies, Long-Term Effect, Double-Stranded DNA, (Deoxyribonucleotide)m, deoxyribonucleic acids, DNAn, Garments, tumor metastasis, Long-Term Effects, plasma, incidence, Cancer, Tumors, Nurse, hereditary prostate cancer, Cerebro Spinal Fluid, cancer of the prostate, Prostates, Frozen Plasma, Malignant Neoplasm, Uniform, DNAn+1, Metastases, frequency, familial, disorders, Fluids, Longterm Effect, Cerebrospinal Fluids, spinal fluid, medical condition, Double-Stranded, Blood Serum, Plasmas, Client, results, Cell, Spinal Fluid, metastatic neoplasm, Cerebro Spinal Fluids, (Deoxyribonucleotide)n+m, Metastase, Frozen Plasmas, Spinal Fluids, metastatic disease, Nurse Uniforms, Benign, Fluid, male prostate, Prostatic Cancer, Long Term Effects, chemical analysis, Diseases, Neoplasm, condition, NOS, ds-DNA, desoxyribose nucleic acid, outbreaks, Plasma, Body Fluid, Garment, Deoxyribonucleic acids., School, Benign Neoplasms, portion of plasma, common, Cancers, CSF, Cerebro, cancer of prostate, surveillance, morbidity, endemics, Body, Prostate Neoplasm, Longterm Effects, Malignant Neoplasms, disease, metastatic, School Uniforms, Patient, School Uniform, metastatic tumor, ds DNA, metastatic tumour, Uniforms, Desoxyribonukleinsaeure, prostate cancer, PC, epidemics, assay, DNA, Clothes, other neoplasm, Prostate Cancer, prostate, Neoplasia, Serums, Prostate, prostata"],"additional_accession":[]},"is_claimable":false,"name":"The evolutionary steps from primary to metastatic prostate cancer are largely uncharted, and the ability to use DNA present in body fluids as correlates of aggregate metastatic status is under-examined. We reconstructed phylogenies in ten prostate cancer patients with fatal disease using deep targeted sequencing of the prostate, adjacent and distant organs, as well as plasma, serum, and cerebrospinal fluid at various time points. A total of 163 samples are studied.","description":"The evolutionary steps from primary tumor to metastasis in prostate cancer are largely uncharted, and the ability to use serum, plasma, and cerebrospinal fluid as a correlate of aggregate metastatic tumor genomic status has not been tested. We used deep targeted sequencing to reconstruct tumor evolution in ten prostate cancer patients with fatal disease encompassing examination of the prostate and adjacent and distant organs, as well as plasma, serum, and cerebrospinal fluid at various time points. We show that there is substantial evolution from a common ancestor within the prostate that results in branching to multiple lineages which form an intermixed multi-clonal primary tumor mass. After the occurrence of key driver aberrations, one of these lineages will metastasize to multiple sites in a sequential fashion. These metastatic sites are then susceptible to being populated by cells from other intra-prostatic lineages or from other metastases. Genomic representation of metastases in body fluids is not uniform. Cerebrospinal fluid analysis can detect lineages not detected in circulating DNA, suggesting possible clinical utility.","dates":{"updated":"2020-08-05 10:44:04"},"accession":"EGAS00001003848","cross_references":{"TAXONOMY":["9606"],"EGA":["EGAD00001005381","EGAD00001005974","EGAD00001005382","EGAC00001001309"]}}