EGAGenomicsOtherhttps://ega-archive.org/studies/EGAS00001003896EGAEGA study EGAS00001003896Methylation_BothEGArestrictedOCSC, measuring, Carcinoma, Squamous Cell Carcinoma of the Mouth, wide/broad, Squamous Cell Carcinoma of the Nasal Cavity, determination, Nucleobase, Methylomes, Squamous Cell Carcinoma of the Head and Neck, Head And Neck, DNA Methylations, broad, Squamous Cell Carcinoma, Squamous Cell Carcinoma of the Larynx, Hypopharyngeal Squamous Cell Carcinoma, microarray., oral squamous cell carcinoma, chemical analysis, Base2, Base1, Oral Squamous Cell Carcinoma, HNSCC, Methylome, Oropharyngeal Squamous Cell Carcinoma, DNA methylation maintenance, Epigenomes, Laryngeal Squamous Cell Carcinoma, bases, Basen, Oral Tongue Squamous Cell Carcinoma, Head And Neck Squamous Cell Carcinomas, DNA methylation, Oral Squamous Cell Carcinomas, DNA Methylomes, wide, Oral Cavity Squamous Cell Carcinoma, scientific observation, Squamous Cell Carcinoma of Larynx, Base, Head and Neck Squamous Cell Carcinoma, assay, nucleobases, DNA, Squamous Cell of Head and Neck, Methylations, Methylation, DNA Methylome, baseORAL NEOPL, neoplasm of tongue, malignant neoplasm of fixed part of tongue, Activity, Oral Neoplasm, multiple endocrine neoplasia syndrome, Laboratory, tumor of the tongue, Head And Neck, malignant neoplasm of dorsal tongue, DNA Methylations, Xkl-1, Squamous Cell Carcinoma, Tumor, Case Fatality Rates, Death Rates, KL receptor activity, Gsfsco1, oral squamous cell carcinoma, tumour of the tongue, SCO5, malignant neoplasm of mobile part of tongue, SCO1, Excess Mortalities, Research Activity, malignant tumour of lingual tonsil, Gsfsow3, Gsfsco5, Laboratory Research, SOW3, Priorities, treatment, average, malignant neoplasm of tip and lateral border of tongue, thymus nucleic acid, Prognoses, DNA methylation maintenance, Man (Taxonomy), TONGUE NEOPL, Genomes, Crude Mortality Rates, tumour of tongue, Tissue, Head And Neck Squamous Cell Carcinomas, W, Oral Squamous Cell Carcinomas, Boys, malignant neoplasm of mobile part of tongue NOS, DNA methylation, malignant neoplasm of base of tongue, malignant neoplasm of dorsal surface of tongue, Estimated, malignant neoplasm of other sites of tongue, tongue tumor, malignant neoplasm, ELL1, CFR Case Fatality Rate, Ell1, sample, disease management, tumor of tongue, Therapies, Bs, Malignancies, Double-Stranded DNA, NEOPL TONGUE, deoxyribonucleic acids, Research Priority, Age-Specific Death Rate, DNAn, Squamous Cell of Head and Neck, Sl, Tongue Cancers, Tumors, single-organism behavior, Therapy, Tongue, Carcinoma, Multiple endocrine neoplasms, wide/broad, PLATEST, DmelCG10120, malignant neoplasm of ventral tongue surface, Process, malignant tumour of mobile part of tongue, Modern, Crude Death, Tr-kit, Research Priorities, malignant neoplasm of tongue, Double-Stranded, familial endocrine, Acceptance Processes, Crude Mortality, NEOPL ORAL, (Deoxyribonucleotide)n+m, malignant tumor of mobile part of tongue, PBT, Acceptance Process, malignant tumor of anterior two-thirds of tongue, Mortality, Benign, malignant neoplasm of border of tongue, Oral Squamous Cell Carcinoma, kl1-A, NOS, simple tissue, malignant neoplasm of tip and/or lateral border of tongue, KIT, multiple endocrine adenomatosis, desoxyribose nucleic acid, Research and Development, tyrosine-protein kinase Kit, Oropharyngeal Squamous Cell Carcinoma, pbt, tongue neoplasm, Factors, death rate, Prognostic, Laryngeal Squamous Cell Carcinoma, Cancer of the Tongue, Adenomatosis, common, Benign Neoplasms, Mortalities, malignant tumor of base of tongue, whole genome, kit, Treatments, human, Malignant Neoplasms, Activities, Endocrine neoplasia, wide, Mortality Rate, Crude Death Rate, multiple, Patient, C19orf17, Republic of India, ds DNA, anon-WO0118547.278, malignant tumour of anterior two-thirds of tongue, DNA, multiple endocrine neoplasia syndrome(s), Mortality Determinant, other neoplasm, Mdh-NADP, Methylation, Platelets, Prognostic Factor, krk1, OCSC, malignant neoplasm of junctional zone of tongue, human being, DNS, (Deoxyribonucleotide)n, Processes, Mortality Declines, Neoplasms, Benign Neoplasm, Excess, SCF receptor activity, men syndromes, Multiple endocrine neoplasia, Prognostic Factors, Crude Mortality Rate, broad, OSCC cell, Malignant, mortality measurement, Oral Neoplasms, malignant neoplasm of the tongue, Deoxyribonucleic acids, gDNA, Age-Specific Death, Human, Mortality Determinants, Crude, Rate, Determinant, Client., Deoxyribonucleic Acid, scfr, Homo sapiens, Case Fatality, malignant tumour of base of tongue, HNSCC, tongue neoplasm (disease), Tongue Neoplasm, SCFR, Cancer of Tongue, Man, Differential Mortality, Fdc, Excess Mortality, Oral Cancer, Malignancy, Oral, Research, malignant tumor of posterior tongue, multiple endocrine neoplasia, Double Stranded, Deoxyribonucleic acid, Age-Specific, OSCC, Neoplasias, men syndrome, malignant neoplasm of anterior 2/3 of tongue, Clients, Mortality Rates, mortality rate, Crude Death Rates, (Deoxyribonucleotide)m, Development and Research, Behaviors, Age Specific Death Rate, Methylations, Cancer, proto-oncogene c-Kit, Squamous Cell Carcinoma of the Mouth, Tongue Cancer, Malignant Neoplasm, Squamous Cell Carcinoma of the Nasal Cavity, Mortality Decline, whole blood, DNAn+1, Multiple Endocrine Neoplasia, MDH, Squamous Cell Carcinoma of the Head and Neck, Factor, ME, Squamous Cell Carcinoma of the Larynx, Client, malignant tumour of posterior tongue, Hypopharyngeal Squamous Cell Carcinoma, Case Fatality Rate, malignant neoplasm of tongue NOS, Priority, MT, survival, Oral Cancers, KIT ligand receptor activity, Familial endocrine adenomatosis, Research Activities, Neoplasm, Tongue Neoplasms, MEN, Men, ds-DNA, Me, malignant neoplasm of dorsum of tongue, tongue neoplasm malignant stage unspecified, Determinants, Death, malignant neoplasm of anterior two-thirds of tongue, XKrk1, neoplasm of the tongue, Rates, Acceptance, tip and lateral border, Death Rate, c-KIT, primary cancer, malignant neoplasm of dorsal tongue NOS, malignant tumor of lingual tonsil, mem, part unspecified, Oral Tongue Squamous Cell Carcinoma, Cancers, men, Age-Specific Death Rates, CD117, malignant tumor, sample population, Differential, c-kit, Oral Cavity Squamous Cell Carcinoma, PPP1R68, Therapeutic, C-Kit, concentration, malignant neoplasm of fixed part of tongue NOS, Ssm, Squamous Cell Carcinoma of Larynx, tongue tumour, Modern Man, Decline, Desoxyribonukleinsaeure, xkl-1, Multiple endocrine adenomatosis, malignant neoplasm of ventral surface of tongue, Treatment, Head and Neck Squamous Cell Carcinoma, Neoplasia, Differential Mortalities, CG10120falseEpigenome wide DNA methylation assay of gingivo-buccal oral squamous cell carcinoma using single base resolution high throughput arrayGingivo-buccal oral squamous cell carcinoma (OSCC-GB) is the most common cancer among men in India and is associated with high mortality. Although OSCC-GB is known to be quite different from tongue cancer in its genomic presentation and its clinical behavior, it is treated identically as tongue cancer. Predictive markers of prognosis and therapy that are specific to OSCC-GB are, therefore, required. To achieve this, we have carried out epigenomic (n=87) profiling of paired tumor-normal tissues collected from OSCC-GB patients from India and genome-wide DNA methylation assays were performed. DNA was isolated using DNeasy Blood and Tissue Kit (Qiagen). The purity and concentration was estimated using NanoDrop 2000 (Thermo Fisher Scientific). Approximately 500 ng genomic DNA from each sample was used for sodium bisulfite conversion using the EZ DNA methylation Gold Kit (Zymo Research, USA). Genome-wide DNA methylation was assayed using iScan (Illumina), for paired tumour and adjacent normal samples of 25 patients using the Infinium Human Methylation450 BeadChip and of 62 patients using the Infinium MethylationEPIC BeadChip.2020-03-09 15:53:52EGAS000010038969606EGAD00010001850EGAC00001000010