EGAGenomicsTranscriptome AnalysisCOVID-19SARS-CoV-22019 novel coronavirus infection2019-nCoV infectionsevere acute respiratory syndrome coronavirus 2coronavirus disease 2019SARS-coronavirus 2https://ega-archive.org/studies/EGAS00001005465EGAEGA study EGAS00001005465Newcastle COVID-19 Single-cell PBMCUCL COVID-19 Single-cell PBMCCambridge COVID-19 Single-cell PBMCrestrictedEGACOVID19, Disease, dTAF[[II]]230, TAF[[II]]250, d230, Coronavirus Disease 19, β-CoV, whole blood, beta-CoV, Blood, COVID-19, TAF200, SARS-CoV-2, l(3)84Ab, Virus Infection, dTAFII250, COVID-19 Virus Diseases, 2019 novel coronavirus, BG:DS00004.13, 2019 Novel Coronavirus Disease, SARS-CoV-2 Infections, TAFII-250, 2019 nCoV Infection, TAF250/230, COVID 19 Virus Infection, EfW1, Virus Disease, Cell, 2019 nCoV Disease, dTAF230, COVID-19 Virus Disease, dmTAF[[II]]230, 2019-nCoV Infection, TAFII250, Client., dmTAF1, Taf230, p230, 2019-nCoV Disease, PBMC, SARS CoV 2 Infection, Infection, TAF[[II]]250/230, Severe Acute 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Healthy Volunteer, d230, Bursa-Dependent, Mortality Declines, SARS Related Coronavirus, COVID-19, Excess, Gene, B-Cell, COVID-19 Virus Diseases, dTAFII250, Crude Mortality Rate, SARS CoV 2 Virus, mortality measurement, EfW1, SARS Coronavirus, COVID19 Virus, Age-Specific Death, Human, Mortality Determinants, Crude, Rate, Determinant, SARS, dmTAF1, Taf230, SARS CoV 2 Infection, Gene Products, Severe Acute Respiratory Syndrome Coronavirus 2 Infection, disease or disorder, Case Fatality, β-coronavirus, 2019-nCoV, 2019 Novel Coronaviruses, TAF250, Differential Mortality, SARS-like coronavirus, Taf200, SARS Coronavirus 2, B-cell, β-CoVs, Excess Mortality, dTAF[[II]]250, Viruses, occurrence, cell, LPS, Bursa Dependent Lymphocytes, COVID-19 Virus Infection, Wuhan Seafood Market Pneumonia Virus, prevalence, severe acute respiratory syndrome coronavirus 2, SARS-like coronaviruses, Taf1p, Age-Specific, SARS-CoV-2 Infection, Non-Polyadenylated RNA, non-neoplastic, dTAF250, COVID-19 Virus, lipopolysaccharides, Immune Response, Severe Acute Respiratory Syndrome Virus, Mononuclear Leukocyte, Clients, Mortality Rates, mortality rate, Control Group, Crude Death Rates, disorder, TAF, Immune Process, Age Specific Death Rate, SARS Coronavirus 2 Infection, Wuhan Coronavirus, incidence, Lymphocyte, Morbidities, Disease, RNA, TAF[[II]]250, Leukocyte, Coronavirus Disease 19, 2019 Novel Coronavirus, Mortality Decline, beta-CoV, Severe acute respiratory syndrome related coronavirus, Proteins, disorders, l(3)84Ab, RNS, BG:DS00004.13, medical condition, SARS-CoV-2 Infections, HCoV-SARS, Client, Cell, dTAF230, COVID-19 Virus Disease, 2019-nCoV Infection, Case Fatality Rate, Severe Acute Respiratory Syndrome Coronavirus 2, survival, p230, yeast nucleic acid, Urbani SARS Associated Coronavirus, chemical analysis, Protein, Infection, TAF[[II]]250/230, TFIID, condition, Determinants, Death, outbreaks, Taf[[II]]250, Rates, ribonucleic acid, Peripheral Blood Human Mononuclear Cells, Death Rate, 2019 Novel, Volunteer, betacoronavirus, Disease 2019, TAF[[II]]230, Bursa-Dependent Lymphocyte, Healthy Subject, Non Polyadenylated RNA, Non-Polyadenylated, Bursa-Equivalent Lymphocyte, Lymphocytes, TAF[II]250, Wuhan, Ribonucleic Acid, SARSr-CoV, Age-Specific Death Rates, COVID-19 Pandemics, COVID-19 Pandemic, endemics, SARS-coronavirus 2, Healthy, Protein Gene Products, Urbani SARS-Associated, Gene Proteins, B-Cells, Differential, DmelCG17603, SARS virus, Decline, Response, SARS Virus, epidemics, COVID 19 Virus, assay, Urbani, SARS Associated Coronavirus, B-lymphocyte, Severe acute respiratory syndrome coronavirus, COVID 19, Severe, Differential Mortalities, TAF1falseMulti-omic single-cell profiling of peripheral blood immune cells from COVID-19 patients and controls.The COVID-19 pandemic, caused by SARS coronavirus 2 (SARS-CoV-2), has resulted in excess morbidity and mortality as well as economic decline. To characterise the systemic host immune response to SARS-CoV-2, we performed single-cell RNA-sequencing coupled with analysis of cell surface proteins, providing molecular profiling of over 800,000 peripheral blood mononuclear cells from a cohort of 130 patients with COVID-19. Our cohort, from three UK centres, spans the spectrum of clinical presentations and disease severities ranging from asymptomatic to critical. Three control groups were included: healthy volunteers, patients suffering from a non-COVID-19 severe respiratory illness and healthy individuals administered with intravenous lipopolysaccharide to model an acute inflammatory response. Full single cell transcriptomes are coupled with quantification of 188 cell surface proteins, and T and B lymphocyte antigen receptor repertoires.2021-08-10 13:37:03EGAS000010054659606MONDO_0100096EGAD00001007867EGAD00001007866EGAD00001007865EGAC00001002221EGAC00001002222EGAC00001002220