{"database":"EGA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"study_type":["RNASeq"],"full_dataset_link":["https://ega-archive.org/studies/EGAS00001005507"],"host":["EGA"],"description":["EGA study EGAS00001005507"],"dataset_title":["Single Cell Transcriptomics of peripheral immune cells pre- and post-Immune Checkpoint Blockade"],"repository":["EGA"],"category":["restricted"],"name_synonyms":["F23A5_3., PRE, dTAF[[II]]230, TAF[[II]]250, d230, PhrB photolyase activity, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, TAF200, l(3)84Ab, dTAFII250, BG:DS00004.13, TAFII-250, photoreactivating enzyme activity, TAF250/230, pigmented epithelium, EfW1, Cell, dTAF230, dmTAF[[II]]230, TAFII250, MODIFIER OF SNC1, retinal pigment, dmTAF1, Taf230, p230, deoxyribonucleic photolyase activity, TAF[[II]]250/230, dipyrimidine photolyase (photosensitive), TFIID, 3, stratum pigmentosa retinae, NUP96, epithelium, retinal pigment layer, TAF250, Taf[[II]]250, Taf200, MOS3, RPE, dTAF[[II]]250, TAF[[II]]230, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, TFIID TAF250, PRECOCIOUS, cel, photolyase activity, cell, pigmented retina, F23A5.3, Taf1p, TAF[II]250, CG17603, TAF[[II]], DNA cyclobutane dipyrimidine photolyase activity, SUPPRESSOR OF AUXIN RESISTANCE 3, p. pigmentosa retinae, dTAF250, phr A photolyase activity, DmelCG17603, DNA-photoreactivating enzyme, Taf250, SR3-5, deoxyribonucleate pyrimidine dimer lyase (photosensitive), TAF, TAF230, TAF1"],"description_synonyms":["MK-3475, CIB8, Bru, lambrolizumab, Thymus-Dependent Lymphocytes, ONO-4538, Raw, determination, AI461847, BMS 936558, Blood, MDX CTLA 4, BMS936558, Gene, MDX-010, T-Lymphocyte, NK/GPI., Monocyte differentiation antigen CD14, DOI, T Lymphocyte, Publication, cromo, urinary form, PBMC, monocyte differentiation antigen CD14, Pgi, Del(8)44H, B8, Gpi-1, MAL, immature T cell, ONO 4538, doi, Svc, treatment, MDX010, MDX-CTLA-4, 24Cr, anatomical systems, Gene Expressions, Gpi-1r, Nlk, T-Cells, Gpi-1s, MDX-1106, Phi, Gpi-1t, T, myeloid cell-specific leucine-rich glycoprotein, Opdivo, CD14, Expressions, MDX 010, ONO4538, T Cells, Monocyte, SCH-900475, mature T cell, disease management, Therapies, Keytruda, Expression, associated, 10X sequencing, metastatic malignant melanoma, T Lymphocytes, Thymus Dependent Lymphocytes, Lymphocyte, MDX1106, Therapy, Gpi, Anti-CTLA-4 MAb, whole blood, Cr, Leu2, 10x sequencing, MF, Amf, Cell, MDX 1106, chrome, mOC-X, T-Cell, Myeloid cell-specific leucine-rich glycoprotein, chemical analysis, membrane-bound form, Anti CTLA 4 MAb Ipilimumab, NK|GPI, metastatic melanoma, Library, T lymphocyte, Anti-CTLA-4 MAb Ipilimumab, NK, T-cell, Org, ORG, Ipilimumab, Col4a-1, Peripheral Blood, Chrom, p32, Thymus-Dependent, Gpi1-r, Lymphocytes, Gpi1-s, Gpi1-t, Treatments, Thymus-Dependent Lymphocyte, Reticuloendothelial System, chromium, T cell, Therapeutic, BMS-936558, PBMCs, Cells, Treatment, T Cell, assay, CD8, Chromium, Bglap-rs1, Yervoy, Gpi1s"],"additional_accession":[]},"is_claimable":false,"name":"Single Cell Transcriptomics of peripheral immune cells pre- and post-Immune Checkpoint Blockade","description":"Raw sequencing data for magnetically-sorted CD8+ T cells and monocytes from the peripheral blood of eight individuals receiving immune checkpoint blockade (either combination ipilimumab/nivolumab, n=4; or single-agent pembrolizumab, n=4) for the treatment of metastatic melanoma. Samples were taken immediately before the first treatment and 21 days post-treatment. Cells were selected based on positivity for CD8 or CD14 and sorted into single cells using the Chromium (10X Genomics) droplet-based system. Gene expression and V(D)J libraries were  prepared in accordance with published protocols before sequencing Illumina HiSeq4000. Analysis of CD8 T cell gene expression and V(D)J features can be found in the associated publication (DOI https://doi.org/10.1101/2020.11.15.383786).","dates":{"updated":"2021-08-19 09:15:30"},"accession":"EGAS00001005507","cross_references":{"TAXONOMY":["9606"],"EGA":["EGAD00001007942","EGAC00001002244"]}}