EGAGenomicsRNASeqhttps://ega-archive.org/studies/EGAS00001005507EGAEGA study EGAS00001005507Single Cell Transcriptomics of peripheral immune cells pre- and post-Immune Checkpoint BlockadeEGArestrictedfalseSingle Cell Transcriptomics of peripheral immune cells pre- and post-Immune Checkpoint BlockadeRaw sequencing data for magnetically-sorted CD8+ T cells and monocytes from the peripheral blood of eight individuals receiving immune checkpoint blockade (either combination ipilimumab/nivolumab, n=4; or single-agent pembrolizumab, n=4) for the treatment of metastatic melanoma. Samples were taken immediately before the first treatment and 21 days post-treatment. Cells were selected based on positivity for CD8 or CD14 and sorted into single cells using the Chromium (10X Genomics) droplet-based system. Gene expression and V(D)J libraries were prepared in accordance with published protocols before sequencing Illumina HiSeq4000. Analysis of CD8 T cell gene expression and V(D)J features can be found in the associated publication (DOI https://doi.org/10.1101/2020.11.15.383786).2021-08-19 09:15:30EGAS000010055079606EGAD00001007942EGAC00001002244