EGAGenomicsOtherhttps://ega-archive.org/studies/EGAS00001006702EGAEGA study EGAS00001006702scRNA Seq of MCSP+ melanoma DCC and bulkRNA Seq of MelDCC linesEGArestrictedMRMV1, CRC18., CRCR1, Igdcc1, C030036D22Rik, malignant, Naevocarcinoma, NTN1R1, snPHGPx, MCSP, HMW-MAA, Malignant Melanomas, NG2, GPx-4, HSMCSGEN1, Malignant, melanoma (disease), Melanomas, DCC, Melanoma, MCSPG, MSK16, melanoma, photoreceptor DCC, Malignant Melanoma, IGDCC1, MEL-CSPG, GSHPx-4, snGPx, malignant melanoma, MCS, PHGPxCRCR1, Node, C030036D22Rik, Single-Cell RNAseq, Sc RNAseq, Activity, Metastasis, limbic encephalitis-neuromyotonia-hyperhidrosis-polyneuropathy syndrome, CRC18, Neoplasms, Single Cell Gene Expression Profiling, Benign Neoplasm, NTN1R1, ScRNA seq, Neoplasm Metastases, neoplasm metastasis, Tumor, Malignant, HSMCSGEN1, cancer metastasis, melanoma (disease), single-cell transcriptome sequencing, MSK16, melanoma, resilient, single-cell RNA-seq, tough, Lymph Nodes, GSHPx-4, Seqs, Cell., DmF2, Analysis, Fs(3)Hor, MAL, PHGPx, multicellular organismal biosynthetic process, Seq, Lymph, lod, strong, tumor cell migration, single-organism biosynthetic process, DmelCG2684, Igdcc1, ScRNAseq, Single-Cell Transcriptome Analysis, Malignancy, Analyses, Naevocarcinoma, metastasis, Single Cell RNAseq, MCSP, Single Cell RNA Seq, Transcriptome Analysis, RNA Seq, NTef2, Dietary Fiber, ScRNA-seq, Neoplasias, MCSPG, malignant neoplasm, Clients, Malignancies, RNA-Seq, tumor metastasis, MCS, Cancer, Tumors, MRMV1, Malignant Neoplasm, Single-Cell RNA-Seq, malignant, Metastases, Single-Cell Gene Expression Profiling, Morvan's fibrillary chorea, scRNA-seq, Leu2, snPHGPx, Malignant Melanomas, NG2, GPx-4, Single Cell Gene Expression Analysis, Morvan's syndrome, Client, Cell, Single-Cell, Single Cell Transcriptome Analysis, Fs(3)Sz11, Metastase, Sc-RNAseq, Melanomas, DCC, single cell RNA sequencing, MT, Benign, Melanoma, photoreceptor DCC, Malignant Melanoma, Neoplasm, sequence, IGDCC1, Single-Cell RNA, primary cancer, Single-Cell Transcriptome Analyses, Exhibit, Lymph Node, MFC, p32, MoS, Benign Neoplasms, patient, Cancers, HMW-MAA, Lds, Single-Cell RNA Seq, malignant tumor, Transcriptome Analyses, primary structure of sequence macromolecule, early, Malignant Neoplasms, Single-Cell Transcriptome, metastatic, Patient, Horka, RNAseq, Nodes, CG2684, Fs(3)Horka, MEL-CSPG, snGPx, malignant melanoma, Morvan syndrome, lymph gland, CD8, General activity, NeoplasiafalseCharacterization of MCSP+ melanoma DCC and MelDCC linesTo dissect the sequence of genotypic and phenotypic progression during early metastatic colonization, we searched for the earliest detectable disseminated cancer cells (DCC) and identified MCSP+ melanoma cells as strong candidates for metastasis founder cells (MFC) in lymph nodes. We isolated MCSP+ DCC from various stages of metastatic colony-formation, performed scRNA-seq and unravelled their phenotypic progression. Further we performed bulk sequencing on patient-derived DCC models (MelDCC) and showed that they exhibit an immunosuppressive activity on CD8 T cells.2023-06-27 16:19:03EGAS000010067029606EGAD00001009662EGAC00001002928