EGAGenomicsEpigeneticshttps://ega-archive.org/studies/EGAS00001007075EGAEGA study EGAS00001007075LFS Germline ProfilingEGArestrictedBlood Cells, leukemia, Blood Cell, Leukocyte, DNA methylation maintenance, Peripheral Blood, whole blood, Blood, DNA Methylations, sarcoma family syndrome of 51 and Fraumeni, DNA methylation, LFS, White Blood Corpuscles, LFS3, LFS1, sarcoma family syndrome of Li and Fraumeni., White Blood Corpuscle, Client, White Blood Cells, Reticuloendothelial System, and adrenal gland), Corpuscle, Patient, Clients, PBMC, PBMCs, White Blood, Corpuscles, SBLA syndrome (sarcoma, Li-Fraumeni-like syndrome, White, Blood Corpuscles, DNA, Li Fraumeni Syndrome, Blood Corpuscle, Methylations, White Blood Cell, Methylation, breastleukemia, DmelCG43720, CG33316, 4-amino-, Genomics, CG42589, Dmel_CG13958, Comparative, Dmel_CG34343, sarcoma family syndrome of 51 and Fraumeni, LFS, Functional Genomics, LFS3, LFS1, Hospital, Client, gDNA, Cyt, 4-Pyrimidinedione, and adrenal gland), method, Structural, CG43720, sarcoma family syndrome of Li and Fraumeni, Functional, 2, 4(1H, CG10662, 2(1H)-Pyrimidinone, C, Ura, CG13964, Dmel_CG10662, Comparative Genomics, 4-amino-2(1H)-pyrimidinone, U, 3H)-pyrimidinedione, Urazil, CG13960, CG13961, study protocol, Children, CG34343, plan specification, CG13958, CG13959, 4-amino-2-hydroxypyrimidine, method used in an experiment., Patient, 4-Dioxopyrimidine, Clients, 3H)-Pyrimidinedione, Isolation of Nuclei TAgged in specific Cell Types, Dmel_CG33316, Dmel_CG13964, SBLA syndrome (sarcoma, Li-Fraumeni-like syndrome, Structural Genomics, Dmel_CG42589, Cytosin, Zytosin, breastfalseDNA methylation of peripheral blood leukocytes from patients with Li-Fraumeni syndromeGenomic DNA (~ 1 μg) from LFS patients was treated using sodium bisulfite (Qiagen) converting unmethylated cytosine to uracil but leaving methylated cytosine intact. These samples were then hybridized to the HumanMethylation450 BeadChip or HumanMethylationEPIC BeadChip from Illumina at the Centre for Applied Genomics (TCAG) at the Hospital for Sick Children using the manufacturer’s recommended protocol.2023-03-23 14:40:36EGAS000010070759606EGAD00010002461EGAC00001003154