EGAGenomicsOtherhttps://ega-archive.org/studies/EGAS00001007358EGAEGA study EGAS00001007358Targeted DNA sequencing on bulk bone marrow and peripheral bloodTARGET-seq+ single-cell transcriptome sequencingTARGET-seq+ single-cell genotypingrestrictedEGAClonal Hematopoiesis of Indeterminate Potential, Inflammatory Response, Clonal Hematopoiesis, Colony Forming Unit, human being, Inflammations, Clonal Cytopenia of Undetermined Significance, Progenitor Cells, Colony-Forming Units, Modern, ICUS Idiopathic Cytopenias of Undetermined Significance, Age-related, Progenitor Cell, Hematopoiesis, Age-related Clonal, Aging, Cell, Human, Colony-Forming Unit, Stem Cell, Homo sapiens, Clonal, CCUS Clonal Cytopenia of Undetermined Significance, Idiopathic Cytopenias of Undetermined Significance, Mother Cells, attenuated, Progenitor, Man, CHIP Clonal Hematopoiesis of Indeterminate Potential, Mother Cell, Senescence, Man (Taxonomy), inflammatory response, Stem, Mother, Colony Forming Units, inflammation, ARCH Age-related Clonal Hematopoiesis, Age-related Clonal Hematopoiesis, human, Biological., Biological Aging, Innate Inflammatory Response, Innate Inflammatory Responses, Modern Man, Cells, ARCH Age related Clonal Hematopoiesis, Innate, Age related Clonal HematopoiesisDNA MTase MmuIIIA, d230, human being, TBRS, Transcriptome Profile, Sequence Determination, Blood, Single-Cell Analyses, M.MmuIIIA, Gene Expression Profile, Gene, dTAFII250, LACS 1, Profiles, Medullary, EfW1, Human, Medullary Hematopoiesis, Mutations, dmTAF[[II]]230, Facl2, Homo sapiens, dmTAF1, Taf230, Marrow, medulla ossea, PBMC, Ayu17-449, Yellow, Analysis, foetal AEDS, Man, TAF250, study, DNA MTase HsaIIIA, Single Cell Analysis, Taf200, dTAF[[II]]250, Man (Taxonomy), mKIAA1546, TFIID TAF250, Analyses, DNA methyltransferase MmuIIIA, Determination, cel, FACS, cell, Profile, E130014J05Rik, Dnmt3a, Taf1p, RGD1311625, Sequence Determinations, Signatures, Sequencing, paired, Red Marrow, LACS1, DNA Sequence Analysis, dTAF250, Expression Signature, fetal antiepileptic drug syndrome, Red, Transcriptomes, Acs, TAF, Sequence Analyses, Acas1, DNA methyltransferase HsaIIIA, dTAF[[II]]230, TAF[[II]]250, Transcriptome, whole blood, 2.1.1.37, Modern, Expression Profiles, KIAA1546, TAF200, l(3)84Ab, Hematopoiesis, DNMT3A2, BG:DS00004.13, TAFII-250, TAF250/230, MDS, Single-Cell, Cell, Determinations, dTAF230, Gene Expression, TAFII250, medulla ossium, p230, DNA Sequencing, Expression Signatures, Gene Expression Signatures, M.HsaIIIA, DNA Sequence Determinations, TAF[[II]]250/230, TFIID, tetraspanin2, Gene Expression Signature, DNA Sequence, Expression Profile, MmuIIIA, DNA sequencing, Transcriptome Profiles, Taf[[II]]250, Yellow Marrow, TAF[[II]]230, Peripheral Blood, medullary bone, medulla of bone, foetal antiepileptic drug syndrome, common, TAF[II]250, Haematopoiesis, CG17603, TAF[[II]], FAC sorting, human, DNA Sequence Determination, Reticuloendothelial System, DNA Sequence Analyses, DmelCG17603, fetal AEDS, TAF1., Taf250, SR3-5, Gene Expression Profiles, Acas, Modern Man, PBMCs, F3P11.18, DNA, Signature, Bone, TAF230, F3P11_18, TAF1falseSelective advantage of mutant stem cells in human clonal hematopoiesis is associated with attenuated response to inflammation and agingIn this study we characterised the consequences of DNMT3A- and TET2-mutations in human clonal haematopoiesis. We screened 195 bone marrow samples for mutations by targeted DNA sequencing, and sequenced 99 paired peripheral blood samples to compare mutation detection between bone marrow and blood. We performed single-cell analysis with TARGET-seq+ on 13 samples (4 controls and 9 clonal haematopoiesis samples), which combines single-cell genotyping for targeted mutations with whole transcriptome sequencing on FACS sorted cells. Single-cell genotyping and transcriptomes were sequenced separately and linked by common single-cell identifiers.2024-06-21 12:37:13EGAS000010073589606EGAD00001011150EGAD00001011083EGAD00001011175EGAC00001003303