{"database":"EVA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Vcf":["ftp://ftp.ebi.ac.uk/pub/databases/eva/PRJEB29974/One_SNP-CAU-20181120.vcf.gz.tbi","ftp://ftp.ebi.ac.uk/pub/databases/eva/PRJEB29974/One_SNP-CAU-20181120.vcf.gz","ftp://ftp.ebi.ac.uk/pub/databases/eva/PRJEB29974/One_SNP-CAU-20181120.accessioned.vcf.gz"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"dataset_type":["Whole Genome Sequencing"],"omics_type":["Genomics"],"submitter":["China Agricultural University"],"instrument_platform":["-"],"species":["Bos Taurus"],"full_dataset_link":["https://www.ebi.ac.uk/eva/?eva-study=PRJEB29974"],"repository":["EVA"],"name_synonyms":["Programs, Manchuria, Sciences, Industrial, Inner Mongolia, Industrial Arts, Mainland China, People's Republic of China, Sinkiang., Arts"],"description_synonyms":["dairy cow, Materials, DNS, (Deoxyribonucleotide)n, number, Mini Exon, Gene, Polymerase Chain Reactions, CG30327, Inverse, aligned, presence, BLAST, Deoxyribonucleic acids, CG11478, Blasts, Inverse PCR, Deoxyribonucleic Acid, cattle, Mini-Exon, Polymerase Chain, DmelCG42257, thymus nucleic acid, hematoblast, Genetic, Genomes, Bos bovis, Inverse Polymerase Chain Reaction, Double Stranded, Deoxyribonucleic acid, analyzer, SLAMF2, Bos Tauurus, oxen, sperm, hemocytoblast, Reaction, 65K, blast, CG42257, domestic cow, Double-Stranded DNA, hematocytoblast, snp, Anchored PCR, (Deoxyribonucleotide)m, deoxyribonucleic acids, DNAn, ejaculate, cg11478, Anchored Polymerase Chain Reaction, cow, MEM-102, mCD48, DNAn+1, aligned to, Double-Stranded, BCM1, Cistrons, Dmel_CG30327., (Deoxyribonucleotide)n+m, count in organism, Seminal Plasma, Bos primigenius taurus, CG6393, Genetic Materials, domestic cattle, BLAST1, ds-DNA, desoxyribose nucleic acid, Genetic Material, PCR, Plasma, Anchored, Dmel_CG6393, Reactions, Nested, Nested Polymerase Chain Reaction, Mini-Exons, Exon, pooled, hCD48, INSDC_feature:gene, whole genome, polymerase chain reaction, seminal fluid, ox, Material, concentration, cardinality, ds DNA, Desoxyribonukleinsaeure, Nested PCR, Cistron, DNA, bovine, Seminal"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"the National Science and Technology Programs of China","description":"We designed and synthesized 23 pairs of primers in the entire exons, and 3 kb of 5′ and 3′ flanking regions of bovine LIPJ gene according to the genomic sequences of UMD3.1.1 (GenBank accession number: AC_000183.1). 44 semen DNAs were pooled to use for the PCR amplifications with the equal concentration (50 ng/µL) for each DNA. All the PCR products were sequenced by ABI3730XL DNA analyzer (Applied Biosystems, Foster, CA, USA). Then, we aligned the sequences with the reference genome (UMD3.1.1) using the BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi), and identified the novel SNP, g.10250120A/G.","dates":{"publication":"2018-11-28"},"accession":"PRJEB29974","cross_references":{"TAXONOMY":["9913"]}}