EVAapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/eva/PRJEB30522/Nellore_BarberoMMD.accessioned.vcf.gzftp://ftp.ebi.ac.uk/pub/databases/eva/PRJEB30522/Nellore_BarberoMMD.vcf.gzftp://ftp.ebi.ac.uk/pub/databases/eva/PRJEB30522/Nellore_BarberoMMD.vcf.gz.tbiprimaryOK200CurationGenomicsUNESP/FCAV - Universidade Estadual "Julio de Mesquita Filho"/ Faculdade de Ciencias Agrarias e VeterinariasIllumina MiSeqBos Indicushttps://www.ebi.ac.uk/eva/?eva-study=PRJEB30522EVATaurine, dairy cow, Beef Cows, Materials, reproductive developmental process, cow, Taurus Cattles, Bos indicus Cattles, Holstein Cow, frequency, Taurus Cattle, Gene, Cattle, Taurine Cattles, Indicine, puberty, Bos indicus, Dairy Cows, Cistrons, Pregnancies, Fs(3)Sz11, Domestic Cows., Bos grunniens, PREGN, cattle, Bos taurus, Bos primigenius taurus, Genetic Materials, domestic cattle, DmF2, Indicine Cattles, Fs(3)Hor, Yaks, Holstein, outbreaks, Genetic Material, Cattles, lod, Yak, DmelCG2684, Genetic, Cows, occurrence, Gestation, Domestic Cow, Dairy Cow, prevalence, Bos bovis, Domestic, Dairy, NTef2, Lds, Taurine Cattle, surveillance, Beef, morbidity, endemics, early, Bos Tauurus, Beef Cow, oxen, Puberties, ox, Material, Cow, Horka, Taurus, CG2684, Fs(3)Horka, Indicine Cattle, Cistron, epidemics, Zebu, domestic cow, Zebus, Bos indicus Cattle, bovine, incidenceTaurine, dairy cow, IGFBP-4ase, Materials, HSN1E, determination, Taurus Cattle, vif, Mini Exon, Fushi tarazu factor homolog 1, A Genes, CG30327, Dairy Cows, CG11478, PREGN, Insulin promoter factor 1, Associations, Embryonal LTR-binding protein, IPF1, Ipf1, PAPPE, 3, NUP96, Fs(3)Hor, Steroid hydroxylase positive regulator, A, Yak, DmelCG42257, DmelCG2684, Cows, PAGEN1, sor Genes, Domestic Cow, Dairy Cow, Q, Bos bovis, Domestic, NTef2, Beef, PLAC3, SUPPRESSOR OF AUXIN RESISTANCE 3, Bos Tauurus, oxen, domestic cow, Zebus, Pregnancy-associated plasma protein E1, CXXC finger protein 9, PRE, Pregnancy-associated plasma protein A, mKIAA0832, Beef Cows, zebu, ELP, Taurus Cattles, OPP, IGF-dependent IGFBP-4 protease, frequency, Cattle, Indicine, Bos indicus, Haplotype, Fs(3)Sz11, Bos taurus, CG6393, Islet|duodenum homeobox 1, Genetic Materials, ERRgamma, Bos taurus indicus, Yaks, Holstein, Genetic Material, ADCADN, Dmel_CG6393, PAPP-A2, vif Genes, Q Genes, UNQ203/PRO229, DNA (cytosine-5-)-methyltransferase 1, STF-1, Embryonal long terminal repeat-binding protein, INSDC_feature:gene, GSF, surveillance, morbidity, early, AY534250, Material, Horka, Taurus, Bos primigenius indicus, CG2684, Fs(3)Horka, Cistron, Zebu, IUF1, Bos indicus Cattle, Genetic Material., bovine, Islet|duodenum homeobox-1, vif Gene, PDX-1, NR3B3, SF-1, Gm210, Gene, PAPP-E, sor Gene, PAPP-A, Pregnancies, Nuclear receptor subfamily 5 group A member 1, DIPLA1, Aim, AIM, IDX-1, cattle, 3.4.24.79, Mini-Exon, animal breed, DmF2, Indicine Cattles, MODY4, Domestic Cows, lod, DNMT1, study, MOS3, Genetic, DNMT1_HUMAN, PRECOCIOUS, occurrence, Mody4, prevalence, F23A5.3, Dairy, Taurine Cattle, Probabilities, Adrenal 4-binding protein, API6, Glucose-sensitive factor, Beef Cow, Cow, 65K, MiSeq, XRG4, CG42257, Dmel_CG30327, snp, Insulin upstream factor 1, A Gene, F23A5_3, incidence, DNA MTase HsaI, DNA (cytosine-5)-methyltransferase 1, 3.4.24.-, cg11478, PAPA, cow, mKIAA1889, Bos indicus Cattles, Indicine cattle, errg, Holstein Cow, DNMT, IUF-1, Taurine Cattles, sor, MCMT, Cistrons, Insulin-like growth factor-dependent IGF-binding protein 4 protease, Bos grunniens, MODIFIER OF SNC1, Bos primigenius taurus, DNA methyltransferase HsaI, ERR3, zebu cattle, chemical analysis, domestic cattle, Q Gene, Somatostatin-transactivating factor 1, outbreaks, PAPPA1, Cattles, Data Base, CXXC9, ASBABP2, Genes, CT-2, IPF-1, Gestation, exonic region, Mini-Exons, Exon, Steroid hormone receptor Ad4BP, Lds, CXXC-type zinc finger protein 9, endemics, Errg, Pregnancy-associated plasma protein A2, ox, pdx-1, Indicine Cattle, epidemics, assay, CLEC2C, m.HsaIfalsePolymorphisms in genes associated with puberty and occurrence of early pregnancy in beef cattleThe early pregnancy probability (OPP) in heifers is a trait of high economic value for Nellore. However, the variability among heifers is not always detected because most breeders do not expose heifers while still young. The aim of this study was identify polymorphisms in the genes XKR-4, ESRRG, PAPP-A, PAPP-A2, STF-1 previously associated with (OPP). Three hundred and eighty heifers were sequenced in the exon regions of the candidate genes with MiSeq? sequencer. Nine polymorphisms were detected in the PAPP-A gene, 9 in the XKR4 gene, 24 in the ESRRG gene, and 49 in the PAPP-A2 gene, these polymorphisms found to have been reported in other races, no polymorphism was found in the SFTPA-1 gene. We also detected 39 new polymorphisms that have not yet been described in bos taurus and bos indicus and are not registered in the NCBI database, and of these 12 polymorphisms were found in the XKR4 gene, 16 in the ESRRG gene and 11 in the PAPP-A2 gene. The association analysis showed that the none of the SNP and haplotype had a significant effect (p <0.05) for OPP in Nellore heifers. This study showed that the PAPP-A and XKR4 and ESRRG and PAPP-A2 genes are polymorphic in the Nelore breed, in the SFTPA-1 gene no polymorphisms were found in the Nellore breed. The influence of these markers on the characteristic occurrence of precocious pregnancy was not verified. Forty-nine new polymorphisms were found in the PAPP-A2, XKR-4 and ESRRG genes.2019-07-02PRJEB305229915