{"database":"EVA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Vcf":["ftp://ftp.ebi.ac.uk/pub/databases/eva/PRJNA1435562/MP-MGH-U3_somatic_standardized_fixed.vcf.gz.csi","ftp://ftp.ebi.ac.uk/pub/databases/eva/PRJNA1435562/UCSD-MGH-U3_somatic_standardized_fixed.vcf.gz","ftp://ftp.ebi.ac.uk/pub/databases/eva/PRJNA1435562/UCSD-MGH-U3_somatic_standardized_fixed.vcf.gz.csi","ftp://ftp.ebi.ac.uk/pub/databases/eva/PRJNA1435562/MP-MGH-U3_somatic_standardized_fixed.vcf.gz"],"Other":["ftp://ftp.ebi.ac.uk/pub/databases/eva/PRJNA1435562/MP-MGH-U3_somatic_standardized_fixed.vcf.csi","ftp://ftp.ebi.ac.uk/pub/databases/eva/PRJNA1435562/UCSD-MGH-U3_somatic_standardized_fixed.vcf.csi"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"dataset_type":["Exome Sequencing"],"omics_type":["Genomics"],"submitter":["Shenzhen University, South China Hospital"],"instrument_platform":["Illumina"],"species":["Homo Sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/eva/?eva-study=PRJNA1435562"],"repository":["EVA"],"additional_accession":[]},"is_claimable":false,"name":"Recurrence in the chemotherapy regimen of bladder carcinoma originates from quiescent epidermoid-like cells","description":"Cancer relapse upon chemotherapy remains the primary challenge in cancer treatment. By integrating high-resolution CRISPR/Cas9-based evolving lineage tracing with single-cell RNA sequencing in mice, we tracked the evolution of bladder carcinoma in the presence or absence of chemotherapy. We found that a cell population exhibiting an epidermoid-like cell state was able to survive chemotherapy. These epidermoid-like cells left the quiescent state to initiate relapse, generating histologically distinct tumors through different evolutionary routes involving specific transcriptional changes. The plasticity of epidermoid-like cells and alterations in plasticity patterns during evolution have shaped the phenotypes of relapsed tumors. Integrated analyses revealed that Insulin-like growth factor-binding protein 5 was a key co-regulator of aggressive progression and squamous features in bladder carcinoma. Impeding Insulin-like growth factor-binding protein 5 expression suppressed the chemoresistance and malignant behaviors of relapsed tumors. Our study deciphers key events and evolutionary dynamics during the natural progression and recurrence of bladder carcinoma. It offers a viable therapeutic approach to overcome the refractoriness of relapsed bladder carcinoma to conventional chemotherapy.","dates":{"publication":"2026-03-12"},"accession":"PRJNA1435562","cross_references":{"TAXONOMY":["9606"]}}