GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE10nnn/GSE10133/primaryOK2000000GenomicsExiguobacterium sibiricum 255-15Expression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE10133GEOGSE0falseArchitecture of thermal adaptation in Exiguobacterium sibiricum strain 255-15: A genome and transcriptome approachSeveral microorganisms have wide temperature growth range and versatility to tolerate large thermal fluctuations in diverse environments. To better understand thermal adaptation of psychrotrophs, Exiguobacterium sibiricum strain 255-15 was used, a psychrotrophic bacterium that grows from -5°C to 39°C. Its genome is approximately 3 Mb in size, has a GC content of 47.7% and includes 2,978 putative protein-encoding genes (CDS). The genome and transcriptome analysis along with the organism's known physiology was used to better understand its thermal adaptation. A total of about 27%, 3.2% and 5.2% of E. sibiricum strain 255-15 CDS spotted on the DNA microarray yielded differentially expressed genes in cells grown at -2.5°C, 10°C and 39°C, respectively, when compared to cells grown at 28°C. The hypothetical and unknown genes represented 10.6%, 0.89% and 2.3% of the CDS differentially expressed when grown at -2.5°C, 10°C and 39°C versus 28°C. The transcriptome analyses showed that E. sibiricum is constitutively adapted to cold temperatures since little differential gene expression was observed at growth temperatures of 10°C and 28°C, but at the extremities of its Arrhenius growth profile, namely -2.5°C and 39°C, much more differential gene expression occurred. The genes that responded were more typically associated with stress response. Keywords: stress response to cold and hot temperatures2008/02/11GSE10133GSM256140GSM256121GSM256143GSM256144GSM256122GSM256141GSM256142GSM256120GSM256136GSM256115GSM256137GSM256134GSM256135GSM256118GSM256119GSM256138GSM256116GSM256139GSM256117GSM256150GSM256132GSM256133GSM256130GSM256131GSM256147GSM256125GSM256148GSM256126GSM256145GSM256123GSM256124GSM256146GSM256129GSM256127GSM256149GSM256128635810133Exiguobacterium sibiricum 255-15[19019206]