GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE11nnn/GSE11892/primaryOK2000000GenomicsHomo sapiensExpression profiling by array; Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11892GEOGSE0falseA Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human TranscriptomeTranscriptome of HEK and B cells were analyzed by microarray and RNA-Seq parallely. Both platforms were then compared in terms of sensitivity. To assess whether values were a reliable indicator of gene activity, we correlated these values with hypophosphorylated RNA polymerase II (PolIIa) occupancy, used as a landmark of transcription initiation. For HEK, we identified PolIIa islands by chromatin immunoprecipitation and sequencing (ChIP-Seq).2008/07/03GSE11892GSM301562GSM301582GSM300493GSM300494GSM301583GSM301581GSM301568GSM301566GSM301567GSM301564GSM30156561049052SRP00071411892Homo sapiens[20150413][18599741]