{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE151nnn/GSE151088/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151088"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"miR-375 Regulates Intestinal Crypt Cell Landscape, Proliferation, and Regenerative Capacity","description":"The role of miRNAs in small intestinal epithelial homeostasis is under-explored. In this study, we discovered that miR-375 is among the most significantly enriched miRNAs in crypts, and in both slowly- and actively-cycling intestinal stem cells (ISCs). We then showed by multiple manipulations, including CRISPR/Cas9 editing, of mouse enteroids that miR-375 is strongly suppressed by Wnt signaling. Single cell RNA-seq analysis of jejunal crypt cells from miR-375 knockout (375-KO) mice revealed elevated numbers of tuft cells and increased expression of Wnt-pathway genes in ISCs. In accordance with these findings, 375-KO mice displayed significant improvement in the clearance of helminth infection and in the regenerative response to whole-body irradiation. The effects of miR-375 on gut epithelial survival and proliferation were further confirmed by ex vivo perturbations in murine enteroids and in vivo over-expression experiments in Drosophila stem/progenitor cells. Functional studies in enteroids revealed that suppression of miR-375 partially compensates for the effects of Yap1 inhibition on cell survival. Consistent with these results, we also find that miR-375 is significantly reduced in mouse and human intestinal tumors. Taken together, this study demonstrates that miR-375 is a master regulator of intestinal epithelial function, proliferation, and regenerative capacity.","dates":{"publication":"2026/05/01"},"accession":"GSE151088","cross_references":{"GSM":["GSM4566200","GSM4566166","GSM4566165","GSM4566201","GSM4566202","GSM4566164","GSM4566163","GSM4566162","GSM4566161","GSM4566160","GSM4566169","GSM4566168","GSM4566167","GSM4566177","GSM4566176","GSM4566175","GSM4566174","GSM4566173","GSM4566172","GSM4566171","GSM4566170","GSM4566179","GSM4566178","GSM4566188","GSM4566187","GSM4566186","GSM4566185","GSM4566184","GSM4566183","GSM4566182","GSM4566181","GSM4566180","GSM4566149","GSM4566189","GSM4566199","GSM4566155","GSM4566154","GSM4566198","GSM4566153","GSM4566197","GSM4566196","GSM4566152","GSM4566151","GSM4566195","GSM4566194","GSM4566150","GSM4566193","GSM4566192","GSM4566191","GSM4566190","GSM4566159","GSM4566158","GSM4566157","GSM4566156"],"GPL":["17021","19057","13112"],"SRA":["SRP263026"],"GSE":["151088"],"taxon":["Mus musculus"]}}