{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE156nnn/GSE156520/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156520"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single cell analysis of de novo infection of permissive cells with MHV-68.","description":"This study sought to define the viral and cellular RNAs expressed within individual cells during de novo, in vitro infection with murine gammaherpesvirus 68 (MHV-68), a small animal model of gammaherpesvirus infection capable of de novo lytic replication in fibroblasts. This analysis focused on virally-infected cells demonstrating viral protein expression, defined through use of a viral fusion protein capable of being detected and purified by fluorescence activated cell sorting. Single cell RNA Seq analysis demonstrated distinct cellular and viral profiles within a permissive fibroblast cell line infected with a uniform virus inoculum and incubation period. These studies reveal: i) pronounced variation in gene expression, with only a small fraction of viral open reading frames uniformly detected in all cells, and a 1000-fold variation in viral RNA expression between cells, ii) positive and negative gene correlations, many uniquely present in a subset of cells, and iii) a dichotomous signature of host gene expression, further revealed by accompanying CyTOF data in the accompanying manuscript.","dates":{"publication":"2026/08/28"},"accession":"GSE156520","cross_references":{"GSM":["GSM4732860","GSM4732861"],"GPL":["24247"],"SRA":["SRP278134"],"GSE":["156520"],"taxon":["Mus musculus"]}}