GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE156nnn/GSE156520/primary200OKTranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156520GEOGSEfalseSingle cell analysis of de novo infection of permissive cells with MHV-68.This study sought to define the viral and cellular RNAs expressed within individual cells during de novo, in vitro infection with murine gammaherpesvirus 68 (MHV-68), a small animal model of gammaherpesvirus infection capable of de novo lytic replication in fibroblasts. This analysis focused on virally-infected cells demonstrating viral protein expression, defined through use of a viral fusion protein capable of being detected and purified by fluorescence activated cell sorting. Single cell RNA Seq analysis demonstrated distinct cellular and viral profiles within a permissive fibroblast cell line infected with a uniform virus inoculum and incubation period. These studies reveal: i) pronounced variation in gene expression, with only a small fraction of viral open reading frames uniformly detected in all cells, and a 1000-fold variation in viral RNA expression between cells, ii) positive and negative gene correlations, many uniquely present in a subset of cells, and iii) a dichotomous signature of host gene expression, further revealed by accompanying CyTOF data in the accompanying manuscript.2026/08/28GSE156520GSM4732860GSM473286124247SRP278134156520Mus musculus