{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE156nnn/GSE156921/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156921"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional effects of activation stimuli on M-CSF-derived macrophages","description":"Analysis of the effect of various activation stimuli on the transcriptional signature of human M-CSF-dependent monocyte-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes from three independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere with 5% CO2 and 21% O2 for 7 days in the presence of 10 ng/ml M-CSF, with cytokine addition every two days. After 7 days, macrophages were either untreated (CNT) or treated with 10 ng/ml E. coli 055:B5 lipopolysaccharide (LPS), 100 ng/ml of the TLR7 agonist CL264 (CL), BSA or 200 mM palmitate (PAL) for 0.5h, 2h, 4h or 12h.","dates":{"publication":"2020/12/09"},"accession":"GSE156921","cross_references":{"GSM":["GSM4748228","GSM4748229","GSM4748226","GSM4748227","GSM4748224","GSM4748225","GSM4748222","GSM4748189","GSM4748223","GSM4748220","GSM4748187","GSM4748188","GSM4748221","GSM4748185","GSM4748186","GSM4748183","GSM4748184","GSM4748181","GSM4748182","GSM4748180","GSM4748239","GSM4748237","GSM4748238","GSM4748235","GSM4748236","GSM4748233","GSM4748234","GSM4748231","GSM4748198","GSM4748199","GSM4748232","GSM4748196","GSM4748197","GSM4748230","GSM4748194","GSM4748195","GSM4748192","GSM4748193","GSM4748190","GSM4748191","GSM4748208","GSM4748209","GSM4748206","GSM4748207","GSM4748204","GSM4748205","GSM4748202","GSM4748203","GSM4748200","GSM4748201","GSM4748242","GSM4748240","GSM4748241","GSM4748219","GSM4748217","GSM4748218","GSM4748215","GSM4748216","GSM4748213","GSM4748214","GSM4748211","GSM4748212","GSM4748210"],"GPL":["23227"],"SRA":["SRP279085"],"GSE":["156921"],"taxon":["Homo sapiens"],"PMID":["[37040733]","[33312178]","[34670213]"]}}