GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE156nnn/GSE156921/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE156921GEOGSEfalseTranscriptional effects of activation stimuli on M-CSF-derived macrophagesAnalysis of the effect of various activation stimuli on the transcriptional signature of human M-CSF-dependent monocyte-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes from three independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere with 5% CO2 and 21% O2 for 7 days in the presence of 10 ng/ml M-CSF, with cytokine addition every two days. After 7 days, macrophages were either untreated (CNT) or treated with 10 ng/ml E. coli 055:B5 lipopolysaccharide (LPS), 100 ng/ml of the TLR7 agonist CL264 (CL), BSA or 200 mM palmitate (PAL) for 0.5h, 2h, 4h or 12h.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 sapiens[37040733][33312178][34670213]