GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE1nnn/GSE1595/primaryOK2000000GenomicsHomo sapiensExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE1595GEOGSE0falseHuman bladder smooth muscle cells - effect of stretch in vitroThe aim of the experiment was to determine the effect of cyclic stretch-relaxation ("stretch") on gene expression patterns in normal diploid human bladder smooth muscle cells. Cells plated on silicone elastomer bottomed 6-well culture dishes were grown to ~80% confluence, serum-depleted for 48h and subjected to cyclic stretch-relaxation at 20% elongation for 4h. Cells seeded in stretch plates but not subjected to stretch served as controls. Total RNA was extracted from both groups of cells, reverse-transcribed, biotin-labeled, fragmented and hybridized to HG-U133A. Four biological replicates were generated for each treatment group (non-stretched or stretched). Keywords = bladder Keywords = smooth muscle cells Keywords = cyclic stretch-relaxation Keywords: other2004/11/04GSE1595GSM27372GSM27373GSM27374GSM27367GSM27368GSM27369GSM27370GSM27371961595Homo sapiens[15467014]