GEOGenomicsOryza sativaNon-coding RNA profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE174230GEOGSEfalseLodiculeless Spikelet is a Mutation of MicroRNA172b Controlling Cleistogamy in RiceCleistogamy is a useful agronomic trait to prevent gene flow in transgenic crops and to maintain genetic purity in autogamous crops including rice. Previously, we isolated a natural cleistogamous rice mutant, lodiculeless spikelet (ld). Map-based cloning approach identified about 4 kb deletion spanning two microRNAs, the entire MIR806a precursor and upstream of MIR172b precursor, in the ld locus. CRISPR/Cas9-mediated transgenic approach validated that mutations of MIR172b, not MIR806a, was responsible for the cleistogamy phenotype. Small RNA sequencing revealed that the expression of miR172b was almost completely reduced in the ld mutants. Quantitative RT-PCR analysis indicated that expression of AP2 target genes of miR172b was not changed in the ld mutants. Finally, we discussed possible regulatory mechanisms on how the defect of MIR172b causes cleistogamy in rice. This is the first report that mutation of a MIR gene causes cleistogamy in rice.2026/05/01GSE174230GSM5289675GSM5289674GSM528967320819SRP319350174230Oryza sativa