GEOTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE181313GEOGSEfalseTranscriptional effects of LXR on the differentiation of monocyte-derived macrophages by tumor ascitic fluids or rheumatoid arthritis synovial fluidsAnalysis of the role of LXR on the transcriptional signature of monocyte-derived macrophages generated in the presence of tumor ascitic fluids or rheumatoid arthritis synovial fluids. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes from independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were treated with DMSO (vehicle), 1 micromolar GW3965 or 1 micromolar GSK2033 for 1 hour, and then cultured in RPMI 1640 10% FBS supplemented with either 50% Tumor-derived Ascitic Fluid (TAF) or 20% Synovial Fluid from patients with active Rheumatoid Arthritis (RASF), at 37°C in a humidified atmosphere with 5% CO2 and 21% O2. After 72 hours, cells were lysed and RNA isolated for transcriptional analysis.2022/03/16GSE181313GSM5494649GSM5494665GSM5494654GSM5494666GSM5494655GSM5494663GSM5494652GSM5494653GSM5494664GSM5494658GSM5494659GSM5494656GSM5494667GSM5494668GSM5494657GSM5494650GSM5494661GSM5494651GSM5494662GSM549466023227SRP330860181313Homo sapiens[36930354][35280993]