GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE18nnn/GSE18488/primaryOK2000000Genomics Schizosaccharomyces pombeSaccharomyces cerevisiaeExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE18488GEOGSE0falseYeast expression data from conditions that inhibit sirtuinsSir2 is an NAD+-dependent histone deacetylase, and is the founding member of a large, phylogentically conserved, family of such deacetylases called the Sirtuins. The budding yeast, Saccharomyces cerevisiae, harbors 4 paralogs of Sir2, known as Hst1, Hst2, Hst3, and Hst4. Reducing the intracellular NAD+ concentration is inhibitory for the Sirtuins, and raising the intracellular nicotinamide (NAM) concentration is inhibitory. Microarray gene expression analysis was used to identify novel classes of yeast genes whose expression is altered when either NAD+ concentration is reduced or NAM is elevated. A subset of genes involved in thiamine biosynthesis was identified as being upregulated when Sir2 or Hst1 was inactivated. Several mutants in the NAD+ biosynthesis and salvage pathways were tested, along with WT and WT grown in the presence of 5 mM nicotinamide, which inhibits the Sirtuins.2010/05/11GSE18488GSM460268GSM460279GSM460267GSM460278GSM460277GSM460266GSM460276GSM460275GSM460274GSM460273GSM460272GSM460269GSM460271GSM460270252918488 Schizosaccharomyces pombeSaccharomyces cerevisiae[20439498]