GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE193nnn/GSE193872/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE193872GEOGSEfalseTranscriptomic analysis of ZHX3-depleted HepG2 cellsThe aim of this RNA-Seq analysis was to identify target genes of the transcription repressor ZHX3 in HepG2 cells. To do so, four stable lines were generated in HepG2 using three different ZHX3-targeting CRISPR constructs and an empty vector construct. Bulk RNA-Seq analysis was performed on these four lines. Sequencing was done by NovogeneAIT Genomics using the Illumina® NovaSeq6000-PE150 platform and 63-82 million base pairs were sequenced per sample. A total of 47 and 156 genes were found to be consistently upregulated and downregulated (fold change ≥ 2 and FDR < 0.05), respectively, in all three KO lines as compared to the empty control line. Functional enrichment analysis of the 156 upregulated genes using g:profiler revealed the enrichment of two biological processess, urate transport and urate metabolic process. qPCR analysis validated the upregulation of uric acid transporter gene SLC17A1.2025/02/26GSE193872GSM5822839GSM5822829GSM5822837GSM5822838GSM5822831GSM5822832GSM5822840GSM5822830GSM5822835GSM5822836GSM5822833GSM582283424676193872Homo sapiens[39990763]