{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE196nnn/GSE196617/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Homo sapiens"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE196617"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Synthetic transcription factor overrides persistent repressive chromatin marks [ChIP-seq]","description":"The prevailing paradigms of epigenetic control posit that signature patterns of post-translational marks on histones guide specific transcriptional outcomes. Methylation of lysine-9 residue of histone H3 (H3K9me3) is implicated in transcriptional silencing through chromatin condensation by its reader HP-1 proteins. Erosion, erasure and subsequent replacement of H3K9me3 by active acetyl-lysine marks accompanies transcriptional up-regulation. Here, we report that a synthetic transcription elongation factor (SynTEF1), selectively targets the disease-causing GAA repeat expansion in frataxin and stimulates gene expression without erasure of the signature repressive marks or removal of HP1 proteins. Added epigenetic perturbation with a pharmacological agent leads to supra-synergistic frataxin expression that is unexpectedly accompanied by a dramatic rise in H3K9me3 levels. Active placement of the signature repressive mark is readily overridden by SynTEF1, demonstrating the fluidity and context-dependence of these marks in regulating gene expression. While forcing a reconsideration of how signature epigenetic marks are interpreted, our results provide key insights for therapeutic intervention in rationally reconfiguring disease-associated epigenetic states.","dates":{"publication":"2026/07/02"},"accession":"GSE196617","cross_references":{"GSM":["GSM9853484","GSM9853482","GSM9853483","GSM9853480","GSM9853481","GSM5896907","GSM5896906","GSM9853428","GSM5896905","GSM5896904","GSM9853429","GSM5896903","GSM9853426","GSM5896902","GSM9853427","GSM5896901","GSM9853424","GSM9853425","GSM5896900","GSM9853422","GSM9853423","GSM9853421","GSM9853439","GSM9853437","GSM9853438","GSM9853435","GSM9853436","GSM9853433","GSM9853434","GSM9853431","GSM9853432","GSM9853430","GSM9853448","GSM9853449","GSM9853446","GSM5896889","GSM9853447","GSM5896888","GSM9853444","GSM9853445","GSM5896887","GSM5896886","GSM9853442","GSM9853443","GSM5896885","GSM5896884","GSM9853440","GSM5896883","GSM9853441","GSM5896882","GSM5896881","GSM5896880","GSM5896915","GSM9853459","GSM5896914","GSM5896913","GSM5896912","GSM9853457","GSM5896879","GSM5896878","GSM9853458","GSM5896911","GSM5896910","GSM9853455","GSM5896877","GSM5896876","GSM9853456","GSM5896875","GSM9853453","GSM9853454","GSM5896874","GSM9853451","GSM5896873","GSM5896872","GSM9853452","GSM9853450","GSM5896909","GSM5896908","GSM9853468","GSM9853469","GSM9853466","GSM9853467","GSM9853464","GSM9853465","GSM9853462","GSM9853463","GSM9853460","GSM9853461","GSM9853479","GSM9853477","GSM5896899","GSM5896898","GSM9853478","GSM9853475","GSM5896897","GSM5896896","GSM9853476","GSM5896895","GSM9853473","GSM9853474","GSM5896894","GSM9853471","GSM5896893","GSM5896892","GSM9853472","GSM5896891","GSM5896890","GSM9853470"],"GPL":["24676"],"GSE":["196617"],"taxon":["Homo sapiens"]}}