GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE197nnn/GSE197886/primaryOK200GenomicsParhyale hawaiensisGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE197886GEOGSEfalseIdentification and classification of cis-regulatory elements in the amphipod crustacean Parhyale hawaiensisHere, we present new functional genomic resources for the amphipod crustacean Parhyale hawaiensis, facilitating the exploration of gene regulatory evolution using this emerging research organism. We use Omni-ATAC-Seq, an improved form of the Assay for Transposase-Accessible Chromatin coupled with next-generation sequencing (ATAC-Seq), to identify accessible chromatin genome-wide across a broad time course of Parhyale embryonic development. This time course encompasses many major morphological events, including segmentation, body regionalization, gut morphogenesis, and limb development. In addition, we use short- and long-read RNA-Seq to generate an improved Parhyale genome annotation, enabling deeper classification of identified regulatory elements. We leverage a variety of bioinformatic tools to discover differential accessibility, predict nucleosome positioning, infer transcription factor binding, cluster peaks based on accessibility dynamics, classify biological functions, and correlate gene expression with accessibility.2022/05/25GSE197886GSM5931712GSM5931711GSM5931697GSM5931696GSM5931710GSM5931699GSM5931698GSM5931693GSM5931692GSM5931695GSM5931694GSM5931691GSM5931690GSM5931709GSM5931708GSM5931705GSM5931704GSM5931707GSM5931706GSM5931701GSM5931689GSM5931700GSM5931703GSM5931702GSM5931686GSM5931685GSM5931688GSM5931687GSM5931684GSM593168332017197886Parhyale hawaiensis