GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE202nnn/GSE202273/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE202273GEOGSEfalseEffects of Smurf1/2 on mouse ESC embryoid body formation and differentiationTo examine the effects of Smurf1 and Smurf2 on embryonic stem cell (ESC) differentiation, we generated mouse ESC lines with Smurf2(flox/flox) (WT control) and Smurf1-/-;Smurf2(∆/∆) (DKO) allelles. These two lines were plated to form embryoid bodies (EBs). We found that Smurfs DKO EBs exhibit distinct morphological differences from WT EBs. Depletion of Smurf1 and Smurf2 delays the embryonic development and arrest cells at gastrulation stage due to heightened Nodal/Smad2 signaling in the process.2026/09/28GSE202273GSM6107602GSM6107601GSM6107604GSM6107603GSM6107598GSM6107597GSM6107600GSM6107599GSM6107606GSM6107605GSM6107608GSM6107607GSM6107594GSM6107593GSM6107596GSM610759513112202273Mus musculus