GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE20nnn/GSE20421/primaryOK2000000GenomicsMultiomics Drosophila sechellia Drosophila melanogaster x Drosophila sechelliaDrosophila melanogasterExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE20421GEOGSE0falseRegulatory Divergence in Drosophila revealed by mRNA-seqThese data were generated in a study to analyze the genetic causes of gene regulatory divergence. mRNA-seq libraies were prepared from poly(A)+ RNA prepared from whole F1 hybrids of D. melanogaster and D. sechellia (female 0-3d post eclosion, "D_mel_x_D_sec"). A mixed mRNA- seq library was prepared from D. melanogaster and D. sechellia poly(A) + RNA (mixed before library preparation, "D_mel_+_D_sec). Finally, separate mRNA-seq libraries were prepared from poly(A)+ RNA from each species ("D_mel" and "D_sec"). The results of this study showed that 78% of genes expressed in the two species are differentially expressed, and that cis- and trans-regulatory divergence affect 51% and 66% of expressed genes, respectively. Keywords: RNA-Seq2010/02/20GSE20421GSM512098GSM512099GSM512096GSM5120971007110070906110069SRP00197620421 Drosophila sechellia Drosophila melanogaster x Drosophila sechelliaDrosophila melanogaster[20354124]