{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE222nnn/GSE222656/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":[" Genome binding/occupancy profiling by high throughput sequencing","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE222656"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq and Cut & Tag analyses of mouse 2-cell embryos in which lamin B1 dissociation from the nuclear envelope is inhibited","description":"We have found that lamin B1 is dissociated from the nuclear envelope transiently at the 2-cell stage during mouse embryonic development. This lamin B1 dissociation is mediated by autophagy and can be inhibited by interfering with lamin B1’s binding to LC3, a factor responsible for early autophagosome formation. In order to block interaction between lamin B1 and LC3, we injected mRNA harboring the LC3-binding domain of lamin B1 to mouse embryos and examined transcriptomes of the injected embryos by RNA sequencing (RNA-seq) analysis. In addition, embryos expressed with full-length lamin B1 mRNA harboring two different types of mutations (flLB1(F454A/L457A) and flLB1(5A)) were analyzed by RNA-seq, together with non-injection control (Non2). Another injection control (flLB1(F454A/L457A)ΔC) was also performed, together with a non-injection control (Non3). Furthermore, the effect of nuclear stiffening was examined by expressing the molecular tether-anchor system (tWT), together with a mutant carrying LaG165A (tMut) and a non-injection control (Non4). Chromatin states of such embryos were analyzed by Cut & Tag using anti-H3K4me3 antibody.","dates":{"publication":"2026/08/08"},"accession":"GSE222656","cross_references":{"GSM":["GSM9843101","GSM8599434","GSM9843102","GSM9843103","GSM9843104","GSM9843105","GSM8599439","GSM9843106","GSM9843107","GSM9843108","GSM9843109","GSM8599435","GSM8599436","GSM8599438","GSM6928376","GSM6928377","GSM6928378","GSM6928379","GSM8599442","GSM9843110","GSM8599443","GSM9843111","GSM8599444","GSM9843112","GSM8599445","GSM9843113","GSM9843114","GSM8599440","GSM9843115","GSM8599446","GSM8599447","GSM8599448","GSM6928387","GSM6928380","GSM6928381","GSM6928382","GSM6928383","GSM6928384","GSM6928385","GSM6928386"],"GPL":["28457","30172"],"GSE":["222656"],"taxon":["Mus musculus"]}}