GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE229nnn/GSE229107/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE229107GEOGSEfalseG-CSF mobilized apheresis as an alternative source of CAR T-cellsCellular therapies such as chimeric antigen receptor-redirected T cells (CART cells) are typically manufactured from an unmanipulated apheresis product collected from the patient or from an allogeneic donor. Whether CART cells can be manufactured from recombinant human granulocyte colony stimulating factor (rhG-CSF) mobilized products, such as those that are cryopreserved for a planned hematopoietic stem cell transplant (HSCT), remains unknown. Production of CART cells from G-CSF mobilized material would be particularly beneficial in settings where CART cells are paired with a HSCT platform, including in the context of acute myeloid leukemia (AML) as we have recently shown. We therefore compared the phenotype and function of CAR T-cells manufactured from paired samples obtained from blood before or after rhG-CSF exposure in healthy volunteer donors, to test the hypothesis that rhG-CSF mobilized CAR T-cells (mobCAR) are functionally equivalent to steady state CAR T-cells (ssCAR). The products were compared using a broad range of phenotypic and functional analytics in vitro and then tested in an in vivo xenograft model, focusing on anti-CD33 CAR T cells for the treatment of AML. CART33 products were successfully manufactured from all four donors. We found mobCART33 equivalent to ssCART33 across all domains tested, and in fact inter-donor variability usually exceeded the intra-donor inter-condition variability. This work supports the use of G-CSF exposed T-cells as a novel starting product for CAR T-cell manufacture, paving the way to the manufacture of an adjunct cell therapy to allogeneic hematopoietic stem-cell transplant from a single apheresis, and providing a means by which previously cryopreserved mobilized units may be utilised.2026/07/01GSE229107GSM7152513GSM7152512GSM7152511GSM7152510GSM7152509GSM7152508GSM7152507GSM715250624676229107Homo sapiens