GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE23nnn/GSE23199/primaryOK2000000GenomicsEscherichia coli Bacillus subtilis subsp. subtilis str. 168Genome binding/occupancy profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE23199GEOGSE0falseChromatin immunoprecipitation of Rok-myc and Hbs-myc in Bacillus subtilisThe Rok protein of Bacillus subtilis was identified as a negative regulator of competence development. Here we show that Rok binds to extended areas of the B. subtilis genome that are characterized by a high A+T content and are known or believed to have been acquired by horizontal gene transfer, e.g. mobile elements. A deletion of rok results in higher excision of one such element, ICEBs1. The preferential association of Rok with DNA with a high A+T content is also observed in a Gram-negative host, E. coli, and depends on a conserved C-terminal region of the protein. Based on our findings, we propose that Rok is a nucleoid-associated protein that fulfills a function analogous to H-NS, a protein absent from most Gram-positive bacteria.2011/01/01GSE23199GSM570847GSM570848GSM570846GSM570849GSM570850GSM5708511070723199Escherichia coli Bacillus subtilis subsp. subtilis str. 168[21085634]