GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE237nnn/GSE237410/primary200OKTranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE237410GEOGSEfalseMyD88 Signaling in Trophoblasts is Necessary for Maternal High Fat Diet-Associated Developmental Programming of ObesityMaternal obesity contributes to persistent programming of offspring obesity. A causal role for placental inflammatory mechanisms in developmental programming remains to be established. We examined the role of the toll-like receptor (TLR) pathway in mediating maternal obesity associated programming by trophoblast deletion of the TLR adapter protein, MyD88. Female MyD88fl/fl:Cyp19 Cre+ (MyD88-CKO) at 5 wks of age were provided control (17% fat calories, CON) or high-fat diets (HFD, 45% fat calories) for 12 wk and mated with MyD88fl/fl:Cyp19 Cre-(flox-control) males. Placenta were studied at dpc 17.5. In a subset, offspring were randomized to CON or HFD for 16 weeks. Placental weight and gene expression changes associated with HFD showed MyD88-dependence for pro-inflammatory and lipid metabolism related responses. Male MyD88 flox offspring from HFD-dams fed postnatal HFD (HH) showed an increase in body weight, liver steatosis and gene expression. These changes were absent in MyD88 CKO HH offspring, demonstrating a causal link between placental MyD88 mediated changes and long-term changes. Overall, these findings provide the first unequivocal evidence that placental changes due to maternal HFD are causally related to offspring obesity risk.2026/06/25GSE237410GSM7611141GSM7611152GSM7611153GSM7611142GSM7611150GSM7611140GSM7611151GSM7611160GSM7611138GSM7611149GSM7611139GSM7611158GSM7611147GSM7611159GSM7611137GSM7611148GSM7611156GSM7611145GSM7611157GSM7611146GSM7611143GSM7611154GSM7611144GSM761115519057237410Mus musculus