{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE240nnn/GSE240606/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"," Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE240606"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"MYC and MAX drive the reactivation of the genome after mitosis","description":"Shortly after mitosis, a wave of hyper-transcription reactivates the genome, especially in mouse embryonic stem (ES) cells, where rapid reactivation is essential for self-renewal and pluripotency. While recent work has illuminated how specific groups of genes are reactivated, whether dedicated mechanisms enable the global, efficient and accurate post-mitotic reactivation of the genome remains unknown. Here, we show that the MYC/MAX transcription factors drive post-mitotic genome reactivation in ES cells. Although MYC undergoes extensive phosphorylation and dissociates from chromatin during mitosis, MAX maintains DNA binding capacity and acts as a mitotic bookmarking factor. Combining pharmacological inhibition of MYC/MAX, inducible genetic MAX ablation, mitotic arrest-release assays and cell cycle-resolved single-cell transcriptomics, we establish the indispensable role of MYC/MAX in post-mitotic hyper-transcription. Our findings suggest a novel mitotic bookmarking function for MAX that orchestrates MYC recruitment to foster post-mitotic transcription. These findings hold significant implications for medically relevant contexts where cell proliferation is of paramount importance. We anticipate that the study of mitotic bookmarking by MYC/MAX and of the effects of drugs targeting their interaction in such process will be relevant for our understanding of cancer and its treatments. ","dates":{"publication":"2026/09/28"},"accession":"GSE240606","cross_references":{"GSM":["GSM7703479","GSM7703499","GSM9000249","GSM7703490","GSM9000268","GSM9000267","GSM9000266","GSM9000265","GSM9000264","GSM7703497","GSM9000263","GSM7703498","GSM9000262","GSM7703495","GSM9000261","GSM7703496","GSM9000260","GSM7703493","GSM7703494","GSM7703491","GSM7703492","GSM7703503","GSM7703504","GSM7703501","GSM7703502","GSM7703488","GSM7703489","GSM7703500","GSM9000259","GSM9000258","GSM9000257","GSM9000256","GSM9000255","GSM9000254","GSM9000253","GSM9000252","GSM7703486","GSM7703487","GSM9000251","GSM7703484","GSM9000250","GSM7703485","GSM7703482","GSM7703483","GSM7703480","GSM7703481"],"GPL":["19057","30172","24247"],"GSE":["240606"],"taxon":["Mus musculus"],"PMID":["[42562574]"]}}