GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE243nnn/GSE243084/primary200OKTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE243084GEOGSEfalseHigh-Throughput Screening of Cancer Cluster Metastasis Inhibitors [Human]Extravasation is a pivotal step in cancer metastasis, however, no molecular control of cancer extravasation has yet been achieved because the mechanism behind this process remain poorly understood. Here, we identify Bay 61-3606 as cancer extravasation inhibitor using high throughput drug screening. Bay 61-3606 significantly decreases cancer cluster extravasation, which is associated with higher metastasis potential, in a zebrafish model. This inhibitory effect extends to decreased distant metastasis in a murine lung carcinoma model and a triple-negative breast cancer model. In addition, our RNA-sequencing results reveal the essential role of COL8A1, a cell-adhesion related molecule expressed by the extravasation participating endothelial cells, in cancer cluster extravasation. Bay 61-3606 treatment effectively decreases COL8A1 through JNK signaling pathway, resulting in impaired cancer cluster extravasation. As the infiltration of immune cells is independent on COL8A1, our findings suggest Bay 61-3606 is a potent drug for anti-metastasis therapy.2026/08/26GSE243084GSM7779101GSM7779100GSM7779099GSM777910224676243084Homo sapiens[42616881]