GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE247nnn/GSE247075/primary200OKTranscriptomicsMus musculus OtherExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE247075GEOGSEfalseActivity-dependent expression of Fezf2 regulates inhibitory synapse formationThe function of the cerebral cortex depends on the proper balance of excitation and inhibition within the cortical circuit. The amount of inhibition a given neuron receives is dependent on its neuronal type. Additionally, neurons can regulate the amount of inhibition they receive through activity-dependent regulation of gene expression. We use bulk sequencing of ribosome-associated RNA to show that selector gene Fezf2, which determines the specification of Layer 5 extratelencephalic (ET) projection neurons in utero, is regulated by neuronal activity. Postnatal reduction of Fezf2 expression decreases perisomatic inhibition originating from PV+ basket cells onto ET projection neurons, without affecting other hallmarks of ET neuron identity. We identify the type II Cadherin Cdh22 as a putative effector gene that is regulated both by FEZF2 and by neuronal activity, and removal of which decreases perisomatic inhibition.2026/09/14GSE247075GSM7882942GSM7882946GSM7882945GSM7882944GSM7882943GSM788294717021247075Mus musculus