GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE26nnn/GSE26004/primaryOK2000000GenomicsHomo sapiensExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26004GEOGSE0falseGPM6B regulates osteoblast differentiation and induction of mineralization by controlling cytoskeleton and matrix vesicle release.Analysis of GPM6B silenced osteogenic hMSC at gene expression level. Analysis is showing significant changes in genes involved in cytoskeleton organization and biogenesis. Immunocytochemistry confirms changed distribution of actin filaments and change in shape and in size of focal adhesions upon GPM6B silencing. Moreover, we demonstrated that production and release of ALP-positive matrix vesicles (MVs) was reduced. In conclusion, we identified GPM6B as a novel regulator of osteoblast differentiation and bone formation and thereby demonstrating the significance of cytoskeleton organization for MV production and eventual mineralization.2011/06/01GSE26004GSM638190GSM638180GSM638191GSM638178GSM638200GSM638189GSM638179GSM638183GSM638194GSM638195GSM638184GSM638192GSM638181GSM638182GSM638193GSM638198GSM638187GSM638188GSM638199GSM638185GSM638196GSM638186GSM638197694726004Homo sapiens[21638316]