GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE26nnn/GSE26136/primaryOK2000000GenomicsMus musculusExpression profiling by array; Genome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26136GEOGSE0falseMammalian Dpy-30 regulates genomic H3K4 methylation and is essential for ES cell fate specificationH3K4 methylation is associated with active genes and, along with H3K27 methylation, is part of a bivalent chromatin mark that typifies poised developmental genes in ESCs. However, its functional roles in ESC maintenance and differentiation are not established. Here we show that mammalian Dpy-30, a core subunit of SET1/MLL complexes, biochemically modulates H3K4 methylation in vitro, and directly regulates chromosomal H3K4me3 throughout the mammalian genome. Depletion of Dpy-30 does not affect ESC self-renewal, but significantly alters the differentiation potential of ESCs, particularly along the neural lineage. The differentiation defect is accompanied by defects in gene induction and H3K4 methylation at key developmental loci. Our results provide strong experimental evidence for the hypothesis that H3K4 methylation is an essential functional component of the bivalent mark during activation of developmental genes in ESCs.2011/02/01GSE26136GSM641810GSM641812GSM641811GSM641807GSM641806GSM651193GSM641809GSM651192GSM641808GSM6418056885110029250SRP00620626136Mus musculus[21335234]