GEOOtherHomo sapiensOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE262435GEOGSEfalseIncorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infectionThe initial steps of the human immunodeficiency virus 1 (HIV-1) lifecycle are regulated by cellular RNA-binding proteins (RBPs). To understand the scope of these early host-virus interactions, we developed in virion RNA interactome capture (ivRIC), which allowed the comprehensive and systematic profiling of the proteins that interact with the HIV-1 genomic (g)RNA inside viral particles. ivRIC identified 104 cellular RBPs within the encapsidated HIV-1 ribonucleoprotein, many of which are typically found in the cellular nucleus. Notably, these nuclear RBPs interact with the HIV-1 RBP Rev, suggesting that they associate with HIV-1 gRNA during its nuclear life. Functional assays show that ivRBPs are important for HIV-1, including PURA and PURB, which control viral gene expression and infectivity through interaction with critical sequences in the gRNA. Our characterisation of the composition of the encapsidated ribonucleoprotein of HIV-1 uncovers new host-virus interactions that invokes new mechanisms for controlling HIV-1 infection.2026/04/13GSE262435GSM8169652GSM8169663GSM8169653GSM8169664GSM8169661GSM8169650GSM8169662GSM8169651GSM8169660GSM8169649GSM8169658GSM8169647GSM8169659GSM8169648GSM8169645GSM8169667GSM8169656GSM8169668GSM8169657GSM8169646GSM8169665GSM8169654GSM8169666GSM816965521697262435Homo sapiens[41955099]