GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE265nnn/GSE265947/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE265947GEOGSEfalseDistinct cytokines regulate gene expression and anti-tumor activity in regenerated CD8+ T cells derived-from induced pluripotent stem cells.Adoptive T cell therapy can induce tumor regression in cancer patients. Tumor-specific CD8+ T cells regenerated from induced pluripotent stem cells (iPSCs), termed regenerated cytotoxic T lymphocytes (CTLs), are promising resources for adoptive T cell therapy. However, it remained unclear what kinds of cytokines enhance the anti-tumor activity of regenerated CTLs. In this study, we examined the effects of exogenous cytokines on the characteristics of regenerated CTLs. We found that IL-15- or IL-21-treatment in vitro enhanced the anti-tumor activity of regenerated CTLs, presumably by different mechanisms. IL-15-treated regenerated CTLs showed early-effector-like characteristics, whereas IL-21-treated regenerated CTLs showed both naïve- and effector-like characteristics. These findings provide useful insights for the clinical application of regenerated CTLs in the future.2026/04/02GSE265947GSM8232426GSM8232427GSM8232433GSM8232434GSM8232431GSM8232432GSM8232430GSM8232428GSM823242924676265947Homo sapiens