{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE267nnn/GSE267532/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mycobacterium tuberculosis"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE267532"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Chemically Induced Alanine Auxotrophy of Mycobacterium tuberculosis","description":"Utilizing a novel computational chemogenomics method to discover new antitubercular compounds, a hit compound designated TI-374 was identified with a minimal inhibitory concentration of 540 nM against M. tuberculosis H37Rv. To investigate the mechanism of action, transcriptional profiling of treated cultures was conducted by RNA-seq. Combined with computational chemogenomic target prediction, metabolomic analysis of treated cultures, nutritional suppression experiments, activity in biochemical assays against purified protein, and whole-genome sequencing of resistant mutants, this transcriptomic data is consistent with a novel mechanism of action whereby TI-374 inhibits the alanine aminotransferase annotated as aspC. This activity interferes with alanine biosynthesis, which is required for growth of the pathogen.","dates":{"publication":"2026/09/24"},"accession":"GSE267532","cross_references":{"GSM":["GSM8268363","GSM8268362","GSM8268361","GSM8268364"],"GPL":["34478"],"GSE":["267532"],"taxon":["Mycobacterium tuberculosis"],"PMID":["[42466413]"]}}