{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE267nnn/GSE267768/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":[" Mus musculus","Homo sapiens"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE267768"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Genuine-seq enables genome-wide unbiased identification of off-target cleavage and translocation by CRISPR nucleases","description":"CRISPR-Cas system is one of the most widely used tool kit for genome editing. In practice, CRISPR’s causes chromosomal structural variation due to off-targets, which is a serious genotoxicity, while currently unbiased methods for detecting chromosomal structural variation are largely lacking. Here, we developed Genome-wide ubiased identification of nuclease effects by sequencing (Genuine-Seq), which could detect both off-target sites of CRISPR and chromosomal structural variations caused by off-target effects, including translocations and large deletions. We demonstrated Genuine-seq’s high efficiency in human cells, and compared spCas9 and spRY, emphasizing that spRY can cause tremendous off-target cutting. Using mouse models, we performed in vivo experiments to depict the landscape of off-target sites and chromosomal structural variations caused by CRISPR-Cas editing, which demonstrated the translational value of Genuine-seq in safety assessment of CRISPR-based therapeutics.","dates":{"publication":"2026/09/26"},"accession":"GSE267768","cross_references":{"GSM":["GSM8275420","GSM8275421","GSM8275422","GSM8275423","GSM8275424","GSM8275425","GSM8275426","GSM8275416","GSM8275417","GSM8275418","GSM8275419","GSM8275430","GSM8275397","GSM8275431","GSM8275398","GSM8275399","GSM8275432","GSM8275433","GSM8275434","GSM8275435","GSM8275436","GSM8275437","GSM8275395","GSM8275396","GSM8275427","GSM8275428","GSM8275429","GSM8275441","GSM8275442","GSM8275443","GSM8275400","GSM8275444","GSM8275445","GSM8275401","GSM8275402","GSM8275446","GSM8275403","GSM8275447","GSM8275448","GSM8275404","GSM8275440","GSM8275438","GSM8275439","GSM8275452","GSM8275453","GSM8275410","GSM8275454","GSM8275411","GSM8275455","GSM8275412","GSM8275413","GSM8275414","GSM8275415","GSM8275450","GSM8275451","GSM8275405","GSM8275449","GSM8275406","GSM8275407","GSM8275408","GSM8275409"],"GPL":["34284","34290"],"GSE":["267768"],"taxon":[" Mus musculus","Homo sapiens"]}}