{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE268nnn/GSE268580/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Homo sapiens"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE268580"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Highly multiplexed single cell chromatin regulator and accessibility profiling elucidates chromatin dynamics in human B cell development and oncogenesis, and reveals an adverse prognostic gene expression signature in B lymphoblastic leukemias","description":"Developing B cells undergo successive stages of chromatin reorganization which modulate DNA accessibility to control V(D)J recombination and gene transcription. These epigenetic shifts are mediated by histone modifications (e.g., H3K4me3, H3K27me3), histone-modifying enzymes (e.g., MLL1, LSD1), and chromatin remodelers (e.g., CTCF, DNMT1). We combined highly-multiplexed, multi-omic approaches to capture this choreographed dance – from single cell changes in chromatin modulators, to alterations in histone modifications, to genome-wide chromatin accessibility. This epigenetic map of normal human B cell differentiation enabled us to peer into the early origins of B cell leukemias and lymphomas – specifically, stage-specific chromatin accessibility at sites of recurrent chromosomal translocations and oncogenic transcription factors (TF). It further identified a chromatin state characterized by relative depletion of chromatin structure modulators and high leukemic TF accessibility, enriched in a small cohort of B lymphoblastic leukemias and associated with adverse prognosis.","dates":{"publication":"2026/06/01"},"accession":"GSE268580","cross_references":{"GSM":["GSM8294452","GSM8294451","GSM8294461","GSM8294450","GSM8294460","GSM8294456","GSM8294455","GSM8294454","GSM8294453","GSM8294449","GSM8294459","GSM8294448","GSM8294458","GSM8294447","GSM8294457"],"GPL":["18573"],"GSE":["268580"],"taxon":["Homo sapiens"]}}