{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE269nnn/GSE269263/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE269263"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Profile of mineralocorticoid receptor-dependent transcriptome from the mouse aldosterone-sensitive distal nephron","description":"The mineralocorticoid receptor (MR, Nr3c2) is responsible for aldosterone-regulation of Na+ and K+ balance and blood pressure. We apply RNA-Seq and bioinformatic approaches in isolated tubule segments of MR KO vs. Control mice to understand how aldosterone activates electrogenic Na+- K+ exchange in the aldosterone sensitive distal nephron (ASDN), including connecting tubule and cortical collecting duct tubule. MR-flox/Pax8‐rtTA/LC1 mice were used as a doxycycline (DOX)-inducible Nr3c2 gene KO model. After DOX treatment, four groups were prepared to distinguish between K+ and MR effects: 1) control mice on normal K+ diet (CT-NK) or 2) high K+ diet (CT-HK) and 3) MR knockout mice on normal K+ diet (KO-NK) or 4) low K+ diet (KO-LK). RNA-Seq analysis was carried out in the microdissected connecting tubule and cortical collecting duct tubule segments (5-6 mice per group and ~10 fresh ASDN tubules per mouse). Differential expression (DE) genes were identified (FDR< 0.05) and used for further bioinformatic analyses. DE genes were identified from comparisons of MR KO-NK vs. CT-NK and MR KO-LK vs. CT-HK, respectively. Absence of transcripts on the third exon of Nr3c2 gene confirmed complete disruption of Nr3c2 gene in the MR KO. All known aldosterone-response genes were significantly decreased in MR KO-LK compared to CT-HK. Our data provide a comprehensive MR-dependent transcriptomic profile of the ASDN isolated from the kidney.","dates":{"publication":"2026/06/30"},"accession":"GSE269263","cross_references":{"GSM":["GSM8311145","GSM8311134","GSM8311146","GSM8311135","GSM8311143","GSM8311132","GSM8311144","GSM8311133","GSM8311141","GSM8311130","GSM8311142","GSM8311131","GSM8311140","GSM8311129","GSM8311127","GSM8311138","GSM8311139","GSM8311128","GSM8311147","GSM8311136","GSM8311125","GSM8311137","GSM8311126"],"GPL":["21103"],"GSE":["269263"],"taxon":["Mus musculus"]}}